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Literature summary for 4.4.1.11 extracted from

  • Takakura, T.; Ito, T.; Yagi, S.; Notsu, Y.; Itakura, T.; Nakamura, T.; Inagaki, K.; Esaki, N.; Hoffman, R.M.; Takimoto, A.
    High-level expression and bulk crystallization of recombinant L-methionine gamma-lyase, an anticancer agent (2006), Appl. Microbiol. Biotechnol., 70, 183-192.
    View publication on PubMed
Search for more literature for 4.4.1.11

Application

Application Comment Organism
drug development endotoxins cause fever in humans and must be eliminated from biopharmaceuticals intended for parenteral administration which are produced by Escherichia coli. Endotoxins can be drastically removed by anion-exchange column chromatography. Thus, the combination of crystallization and anion-exchange column chromatography is suitable for purification of the enzyme Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas putida

General Stability

General Stability Organism
the purified enzyme can be stored at -80°C for several months with no significant loss of activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, SDS-PAGE, gel filtration Pseudomonas putida
160000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine + H2O Pseudomonas putida
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54
-
 crystals Pseudomonas putida
57
-
 purified recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + H2O
-
 Pseudomonas putida methanethiol + NH3 + 2-oxobutanoate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE, gel filtration Pseudomonas putida

Synonyms

Synonyms Comment Organism
L-methionine gamma-lyase
-
 Pseudomonas putida
METase
-
 Pseudomonas putida