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Literature summary for 4.4.1.11 extracted from

  • Tokoro, M.; Asai, T.; Kobayashi, S.; Takeuchi, T.; Nozaki, T.
    Identification and characterization of two isoenzymes of methionine gamma-lyase from Entamoeba histolytica: a key enzyme of sulfur-amino acid degradation in an anaerobic parasitic protist that lacks forward and reverse trans-sulfuration pathways (2003), J. Biol. Chem., 278, 42717-42727.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of EhMGL1 in Escherichia coli Entamoeba histolytica
expression of EhMGL2 in Escherichia coli Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
DL-propargylglycine irreversible slow-binding inhibition Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.94
-
L-methionine 36°C, pH 6.8, recombinant EhMGL1 Entamoeba histolytica
1.87
-
DL-homocysteine 36°C, pH 6.8, recombinant EhMGL2 Entamoeba histolytica
1.9
-
L-methionine 36°C, pH 6.8, recombinant EhMGL2 Entamoeba histolytica
3.4
-
DL-homocysteine 36°C, pH 6.8, recombinant EhMGL1 Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Entamoeba histolytica 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
4 * 43300, SDS-PAGE, immunoblot, native and recombinant EhMGL1 form homotetramers Entamoeba histolytica
43700
-
4 * 43700, SDS-PAGE, immunoblot, native and recombinant EhMGL2 form homotetramers Entamoeba histolytica
171000 177000 gel filtration, native and recombinant EhMGL1 Entamoeba histolytica
171000 177000 gel filtration, native and recombinant EhMGL2 Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine + H2O Entamoeba histolytica
-
methanethiol + NH3 + 2-oxobutanoate
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q86D27 isoenzyme 2
-
Entamoeba histolytica Q86D28 isoenzyme 1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant EhMGL1 Entamoeba histolytica
recombinant EhMGL2 Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
recombinant EhMGL1 Entamoeba histolytica
0.44
-
recombinant EhMGL2 Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + H2O 112% of activity with L-methionine Entamoeba histolytica 2-oxobutanoate + NH3 + H2S
-
?
DL-homocysteine + H2O 294% of EhMGL1 activity with L-methionine Entamoeba histolytica 2-oxobutanoate + NH3 + H2S
-
?
DL-homocysteine + H2O
-
Entamoeba histolytica hydrogen sulfide + NH3 + 2-oxobutanoate
-
?
L-cysteine + H2O 160% of EhMGL1 activity with L-methionine Entamoeba histolytica sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O 20% of activity with L-methionine Entamoeba histolytica sulfide + NH3 + pyruvate
-
?
L-methionine + H2O
-
Entamoeba histolytica methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine + H2O 181% of EhMGL1 activity with L-methionine Entamoeba histolytica methanethiol + NH3 + 2-oxobutanoate
-
?
O-acetyl-L-serine + H2O 11% of activity with L-methionine Entamoeba histolytica 2-oxopropanoate + NH3 + acetate
-
?
O-acetyl-L-serine + H2O 34% of EhMGL1 activity with L-methionine Entamoeba histolytica 2-oxopropanoate + NH3 + acetate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 43300, SDS-PAGE, immunoblot, native and recombinant EhMGL1 form homotetramers Entamoeba histolytica
tetramer 4 * 43700, SDS-PAGE, immunoblot, native and recombinant EhMGL2 form homotetramers Entamoeba histolytica

Synonyms

Synonyms Comment Organism
EhMGL1 methionine gamma-lyase isoenzyme 1 Entamoeba histolytica
EhMGL2 methionine gamma-lyase isoenzyme 2 Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Entamoeba histolytica
7.7
-
-
Entamoeba histolytica

pH Range

pH Minimum pH Maximum Comment Organism
5.6 8.2 approx. 14% of maximal activity at pH 5.6, approx. 10% of maximal activity at pH 8.2 Entamoeba histolytica
5.6 8.2 approx. 55% of maximal activity at pH 5.6, approx. 95% of maximal activity at pH 8.2 Entamoeba histolytica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
DL-propargylglycine
-
Entamoeba histolytica

pI Value

Organism Comment pI Value Maximum pI Value
Entamoeba histolytica two-dimensional PAGE, recombinant EhMGL1
-
5.9
Entamoeba histolytica two-dimensional PAGE, native EhMGL1
-
6
Entamoeba histolytica two-dimensional PAGE, recombinant EhMGL2
-
6.5
Entamoeba histolytica two-dimensional PAGE, native EhMGL2
-
6.7