Literature summary for 4.4.1.11 extracted from

Motoshima, H.; Inagaki, K.; Kumasaka, T.; Furuichi, M.; Inoue, H.; Tamura, T.; Esaki, N.; Soda, K.; Tanaka, N.; Yamamoto, M.; Tanaka, H.
Crystal structure of the pyridoxal 5'-phosphate dependent L-methionine gamma-lyase from Pseudomonas putida (2000), J. Biochem., 128, 349-354.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion, protein solution containing 10-20 mg/ml protein in 20 mM sodium phosphate, pH 7.2, 0.5 mM pyridoxal 5'-phosphate and 0.5% 2-mercaptoethanol is equilibrated against a reservoir solution consisting of 15% polyethylene glycol 6000, 250 mM NaCl, 200 mM MES-NaOH, pH 6.5, 0.5 mM pyridoxal 5'-phosphate and 0.5% 2-mercaptoethanol, crystals grow at room temperatur within 1 week, crystals diffract to 1.7 A	Pseudomonas putida

Crystallization (Comment)

Organism

hanging-drop vapor diffusion, protein solution containing 10-20 mg/ml protein in 20 mM sodium phosphate, pH 7.2, 0.5 mM pyridoxal 5'-phosphate and 0.5% 2-mercaptoethanol is equilibrated against a reservoir solution consisting of 15% polyethylene glycol 6000, 250 mM NaCl, 200 mM MES-NaOH, pH 6.5, 0.5 mM pyridoxal 5'-phosphate and 0.5% 2-mercaptoethanol, crystals grow at room temperatur within 1 week, crystals diffract to 1.7 A

Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-methionine + H2O	Pseudomonas putida	-	methanethiol + NH3 + 2-oxobutanoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-methionine + H2O	-	Pseudomonas putida	methanethiol + NH3 + 2-oxobutanoate	-	?

Synonyms

Synonyms	Comment	Organism
MGL	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Pseudomonas putida

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Release 2023.1 (January 2023)