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Literature summary for 4.4.1.11 extracted from
- Properties of L-methionine gamma-lyase from Pseudomonas ovalis (1977), Biochemistry, 16, 100-106.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing, slight decrease in activity | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| carbonyl reagents | - |
Pseudomonas putida | |
| hydroxylamine | - |
Pseudomonas putida |  |
| sulfhydryl inhibitors | - |
Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, x * 48000, disc gel electrophoresis, guanidine hydrochloride | Pseudomonas putida |
| 48000 | - |
x * 40000, x * 48000, disc gel electrophoresis, guanidine hydrochloride | Pseudomonas putida |
| 173000 | - |
sedimentation equilibrium method | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| additional information | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethionine | - |
Pseudomonas putida | ? | - |
? | |
| L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
ir | |
| additional information | - |
Pseudomonas putida | ? | - |
? | |
| S-methyl-L-cysteine + H2O | - |
Pseudomonas putida | pyruvate + NH3 + CH3SH | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| polymer | x * 40000, x * 48000, disc gel electrophoresis, guanidine hydrochloride | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.5-9.0, stable, 5 min | Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | 50°C, 5 min, stable in pH-range | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 4 mol per mol of enzyme | Pseudomonas putida | |
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