Literature summary for 4.3.3.7 extracted from

Kaur, N.; Gautam, A.; Kumar, S.; Singh, A.; Singh, N.; Sharma, S.; Sharma, R.; Tewari, R.; Singh, T.P.
Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa (2011), Int. J. Biol. Macromol., 48, 779-787.

Application

Application	Comment	Organism
drug development	the intracellular enzyme dihydrodipicolinate synthase a potential drug target because it is essential for the growth of bacteria while it is absent in humans	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
gene dapA, DNA and amino acid sequence determination and analysis, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3)	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant DHDPS, free or in complex with inhibitor (S)-lysine, 15 mg/ml protein in 50 mM Tris-HCl, pH 8.5, at 20°C using hanging drop vapour diffusion method, mixing of 0.005 ml protein solution with 0.005 ml well solution containing 30% w/v PEG-3350, 170 mM MgCl2, 70 mM Tris-HCl, pH 8.5, and 6% v/v propylene glycol. Crystals obtained without 6% propylene glycol are soaked in the reservoir containing 20 mg/ml (S)-lysine, X-ray diffraction structure determination and analysis at 2.65-2.85 A resolution	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

purified recombinant DHDPS, free or in complex with inhibitor (S)-lysine, 15 mg/ml protein in 50 mM Tris-HCl, pH 8.5, at 20°C using hanging drop vapour diffusion method, mixing of 0.005 ml protein solution with 0.005 ml well solution containing 30% w/v PEG-3350, 170 mM MgCl2, 70 mM Tris-HCl, pH 8.5, and 6% v/v propylene glycol. Crystals obtained without 6% propylene glycol are soaked in the reservoir containing 20 mg/ml (S)-lysine, X-ray diffraction structure determination and analysis at 2.65-2.85 A resolution

Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
L-lysine	binds at three sites to the enzyme, binding structure, overview	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.17	-	L-aspartate 4-semialdehyde	pH 8.0, 37°C, recombinant His-tagged enzyme	Pseudomonas aeruginosa
0.9	-	pyruvate	pH 8.0, 37°C, recombinant His-tagged enzyme	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Pseudomonas aeruginosa	5622	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate 4-semialdehyde + pyruvate	Pseudomonas aeruginosa	-	(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
no activity in Homo sapiens	-	-	-
Pseudomonas aeruginosa	Q9I4W3	gene dapA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged DHDPS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate 4-semialdehyde + pyruvate	-	Pseudomonas aeruginosa	(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O	-	?
additional information	the active site is formed by residues Thr44, Tyr107 and Tyr133, stereochemically suitable for catalytic function	Pseudomonas aeruginosa	?	-	?

Subunits

Subunits	Comment	Organism
dimer	three-dimensional structure determination shows that DHDPS forms a homodimer which is stabilized by several hydrogen bonds and van der Waals forces at the interface, active site structure, overview. Each monomer is composed of two domains, the N-terminal domain consists of residues 1-224, and forms an 8-fold parallel alpha/beta barrel	Pseudomonas aeruginosa
More	the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
DHDPS	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Pseudomonas aeruginosa