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Literature summary for 4.3.3.7 extracted from

  • Dobson, R.C.; Perugini, M.A.; Jameson, G.B.; Gerrard, J.A.
    Specificity versus catalytic potency: The role of threonine 44 in Escherichia coli dihydrodipicolinate synthase mediated catalysis (2009), Biochimie, 91, 1036-1044.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant T44S expressed in dapA-deficient Escherichia coli AT997r- Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant T44S, crystals are isomorphous to those of the wild-type enzyme, no significant modification in its tertiary or quaternary structure from that of the wild-type enzyme Escherichia coli

Protein Variants

Protein Variants Comment Organism
T44S the active site is intact, returns much but not all activity likely due to the flexibility of Ser44. Increased flexibility in the active site, which appears to facilitate the binding/reaction of substrate analogues Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-lysine no difference in its sensitivity or behaviour with respect to L-lysine when compared to the wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
L-aspartate 4-semialdehyde mutant T44S Escherichia coli
0.92
-
pyruvate mutant T44S Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6L2
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant T44S, no pyruvate added to the crude extract prior to sonication, purified by heat shock and ion-exchange chromatography, 20.7fold with a yield of 123% Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.72
-
crude extract of mutant T44S Escherichia coli
14.52
-
20.7fold purified mutant T44S Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + pyruvate
-
Escherichia coli dihydrodipicolinate + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
DHDPS
-
Escherichia coli
dihydrodipicolinate synthase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.4
-
pyruvate mutant T44S Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
pyruvate mutant T44S Escherichia coli
81
-
L-aspartate 4-semialdehyde mutant T44S Escherichia coli