Cloned (Comment) | Organism |
---|---|
mutant T44S expressed in dapA-deficient Escherichia coli AT997r- | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
mutant T44S, crystals are isomorphous to those of the wild-type enzyme, no significant modification in its tertiary or quaternary structure from that of the wild-type enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T44S | the active site is intact, returns much but not all activity likely due to the flexibility of Ser44. Increased flexibility in the active site, which appears to facilitate the binding/reaction of substrate analogues | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | no difference in its sensitivity or behaviour with respect to L-lysine when compared to the wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-aspartate 4-semialdehyde | mutant T44S | Escherichia coli | |
0.92 | - |
pyruvate | mutant T44S | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6L2 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant T44S, no pyruvate added to the crude extract prior to sonication, purified by heat shock and ion-exchange chromatography, 20.7fold with a yield of 123% | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
crude extract of mutant T44S | Escherichia coli |
14.52 | - |
20.7fold purified mutant T44S | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | - |
Escherichia coli | dihydrodipicolinate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Escherichia coli |
dihydrodipicolinate synthase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
pyruvate | mutant T44S | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
pyruvate | mutant T44S | Escherichia coli | |
81 | - |
L-aspartate 4-semialdehyde | mutant T44S | Escherichia coli |