Cloned (Comment) | Organism |
---|---|
PCR product ligated into pCRBluntII-TOPO and transformed into Escherichia coli One Shot TOP10. The dapA insert then amplified, the product, which now contains NdeI and BamHI restriction-endonuclease sites, ligated into pCR-BluntII-TOPO and transformed into Escherichia coli One Shot TOP10, which enables efficient ligation into an expression vector. DapA and linearized pET-11a ligated with T4 DNA ligase and transformed into Escherichia coli BL21(DE3) | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
in the presence of pyruvate, the hanging-drop vapour-diffusion method, at a resolution of 2.15 A. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.5, b = 124.6, c = 131.0 A, beta = 90.0 | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81WN7 | Sterne strain | - |
Bacillus anthracis Sterne | Q81WN7 | Sterne strain | - |
Purification (Comment) | Organism |
---|---|
by gel filtration, recombinant enzyme in the absence of the substrate pyruvate (with a yield of 36.3%) and presence of the substrate pyruvate (with a yield of 98%) | Bacillus anthracis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
DHDPS purified in the presence of pyruvate yields a greater amount of recombinant enzyme with 22fold greater specific activity compared with the enzyme purified in the absence of substrate | Bacillus anthracis |
1.5 | - |
purified enzyme, recombinant enzyme in the absence of the substrate pyruvate | Bacillus anthracis |
2 | - |
crude extract, recombinant enzyme in the absence of the substrate pyruvate | Bacillus anthracis |
12 | - |
crude extract, recombinant enzyme in the presence of the substrate pyruvate | Bacillus anthracis |
33 | - |
purified enzyme, recombinant enzyme in the presence of the substrate pyruvate | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | - |
Bacillus anthracis | dihydrodipicolinate + 2 H2O | - |
? | |
L-aspartate 4-semialdehyde + pyruvate | - |
Bacillus anthracis Sterne | dihydrodipicolinate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DapA | - |
Bacillus anthracis |
DHDPS | - |
Bacillus anthracis |
dihydrodipicolinate synthase | - |
Bacillus anthracis |