Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Geobacillus kaustophilus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion, to investigate the mechanism of the synthase subunit, crystal structures are obtained for three intermediate states of the Geobacillus stearothermophilus intact PLPS or its synthase subunit. The structures capture the synthase active site at three distinct steps in its complicated catalytic cycle, provide insights into the elusive mechanism, and illustrate the coordinated motions within the synthase subunit that separate the catalytic states | Geobacillus kaustophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
D-ribose 5-phosphate | pH 8.5, 37°C | Geobacillus kaustophilus | |
0.6 | - |
L-glutamine | pH 8.5, 37°C, glutaminase activity | Geobacillus kaustophilus | |
1.06 | - |
D-glyceraldehyde 3-phosphate | pH 8.5, 37°C | Geobacillus kaustophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine | Geobacillus kaustophilus | - |
pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | Q5L3Y2 AND Q5L3Y1 | Q5L3Y2: subunit PdxS, Q5L3Y1: subunit PdxT | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine | - |
Geobacillus kaustophilus | pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? | |
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 | - |
Geobacillus kaustophilus | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? | |
L-glutamine + H2O | - |
Geobacillus kaustophilus | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | the intact enzyme includes 12 synthase and 12 glutaminase subunits | Geobacillus kaustophilus |
Synonyms | Comment | Organism |
---|---|---|
PLP synthase | - |
Geobacillus kaustophilus |
PLPS | - |
Geobacillus kaustophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
D-glyceraldehyde 3-phosphate | pH 8.5, 37°C | Geobacillus kaustophilus | |
0.00017 | - |
D-ribose 5-phosphate | pH 8.5, 37°C | Geobacillus kaustophilus | |
0.001 | - |
L-glutamine | pH 8.5, 37°C, glutaminase activity | Geobacillus kaustophilus |