Cloned (Comment) | Organism |
---|---|
gene btrC2, DNA and amino acid sequence determination, genetic organization, gene btrC2 is not found in the butirosin-biosynthestic gene cluster, sequence comparison, gene complementation of a btrC2 disruptant by Bacillus subtilis gene pdxT, recombinant expression of untagged BtrC2 in Escherichia coli | Niallia circulans |
gene pdxS, DNA and amino acid sequence determination, genetic organization, recombinant expression of untagged protein encoded by pdxS in Escherichia coli | Niallia circulans |
genes pdxS and pdxT, genetic organization, expression of His-tagged PdxT in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a gene btrC2 disruptant mutant strain 4-41, which shows deficiency in growth and antibiotic production, and the addition of pyridoxal to the medium restores growth and short-term antibiotics production | Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Niallia circulans | BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase | ? | - |
? | |
additional information | Bacillus subtilis | the enzyme catalyzes the conversion of ribose 5-phosphate and glyceraldehyde 3-phosphate to pyridoxal 5'-phosphate | ? | - |
? | |
additional information | Niallia circulans SANK 72073 | BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
genes pdxS and pdxT | - |
Niallia circulans | Q8L1A7 | btrC2; gene btrC2 | - |
Niallia circulans | Q8L1A8 | pdxS; gene pdxS | - |
Niallia circulans SANK 72073 | Q8L1A7 | btrC2; gene btrC2 | - |
Niallia circulans SANK 72073 | Q8L1A8 | pdxS; gene pdxS | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase | Niallia circulans | ? | - |
? | |
additional information | the enzyme catalyzes the conversion of ribose 5-phosphate and glyceraldehyde 3-phosphate to pyridoxal 5'-phosphate | Bacillus subtilis | ? | - |
? | |
additional information | BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase | Niallia circulans SANK 72073 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLP synthase | - |
Bacillus subtilis |
PLP synthase | - |
Niallia circulans |
pyridoxal 5'-phosphate synthase | - |
Bacillus subtilis |
pyridoxal 5'-phosphate synthase | - |
Niallia circulans |
General Information | Comment | Organism |
---|---|---|
malfunction | a btrC2 disruptant shows deficiency in growth and antibiotic production, and the addition of pyridoxal to the medium restores growth and short-term antibiotics production | Niallia circulans |
additional information | proteins PdxS and PdxT interact physically and form the PLP synthase, PdxT is a glutamine amidotransferase subunit that donates an amido group from glutamine to the intermediate of the enzyme reaction | Bacillus subtilis |