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Literature summary for 4.3.3.6 extracted from

  • Itagaki, S.; Haga, M.; Oikawa, Y.; Sakoda, A.; Ohke, Y.; Sawada, H.; Eguchi, T.; Tamegai, H.
    Differences in the roles of a glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase between Bacillus circulans and Bacillus subtilis (2013), Biosci. Biotechnol. Biochem., 77, 1481-1485.
    View publication on PubMed
Search for more literature for 4.3.3.6

Cloned(Commentary)

Cloned (Comment) Organism
gene btrC2, DNA and amino acid sequence determination, genetic organization, gene btrC2 is not found in the butirosin-biosynthestic gene cluster, sequence comparison, gene complementation of a btrC2 disruptant by Bacillus subtilis gene pdxT, recombinant expression of untagged BtrC2 in Escherichia coli Niallia circulans
gene pdxS, DNA and amino acid sequence determination, genetic organization, recombinant expression of untagged protein encoded by pdxS in Escherichia coli Niallia circulans
genes pdxS and pdxT, genetic organization, expression of His-tagged PdxT in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information generation of a gene btrC2 disruptant mutant strain 4-41, which shows deficiency in growth and antibiotic production, and the addition of pyridoxal to the medium restores growth and short-term antibiotics production Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Niallia circulans BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase ?
-
 ?
additional information Bacillus subtilis the enzyme catalyzes the conversion of ribose 5-phosphate and glyceraldehyde 3-phosphate to pyridoxal 5'-phosphate ?
-
 ?
additional information Niallia circulans SANK 72073 BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 genes pdxS and pdxT
-
Niallia circulans Q8L1A7 btrC2; gene btrC2
-
Niallia circulans Q8L1A8 pdxS; gene pdxS
-
Niallia circulans SANK 72073 Q8L1A7 btrC2; gene btrC2
-
Niallia circulans SANK 72073 Q8L1A8 pdxS; gene pdxS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase Niallia circulans ?
-
 ?
additional information the enzyme catalyzes the conversion of ribose 5-phosphate and glyceraldehyde 3-phosphate to pyridoxal 5'-phosphate Bacillus subtilis ?
-
 ?
additional information BtrC2 shows no catalytic activity as 2-deoxy-scyllo-inosose (DOI) synthase Niallia circulans SANK 72073 ?
-
 ?

Synonyms

Synonyms Comment Organism
PLP synthase
-
 Bacillus subtilis
PLP synthase
-
 Niallia circulans
pyridoxal 5'-phosphate synthase
-
 Bacillus subtilis
pyridoxal 5'-phosphate synthase
-
 Niallia circulans

General Information

General Information Comment Organism
malfunction a btrC2 disruptant shows deficiency in growth and antibiotic production, and the addition of pyridoxal to the medium restores growth and short-term antibiotics production Niallia circulans
additional information proteins PdxS and PdxT interact physically and form the PLP synthase, PdxT is a glutamine amidotransferase subunit that donates an amido group from glutamine to the intermediate of the enzyme reaction Bacillus subtilis