Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
DELTA 270-301 | mainly monomer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis | Plasmodium falciparum |
DELTA 273-301 | dodecamer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis | Plasmodium falciparum |
DELTA 279-301 | dodecamer, Pdx2 activation, reduced pyridoxal 5'-phosphate synthesis | Plasmodium falciparum |
DELTA 287-301 | dodecamer, Pdx2 activation, precipitates upon addition of glyceraldehyd 3-phosphate | Plasmodium falciparum |
DELTA 293-301 | behaviour like DELTA 287-301 | Plasmodium falciparum |
DELTA 295-301 | behaviour like DELTA 287-301 | Plasmodium falciparum |
S270A/DELTA 273-301 | dodecamer | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS | Plasmodium falciparum |
363000 | - |
analytical ultracentrifugation, 420000 calculated | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | C6KT50 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), gel filtration | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00025 | - |
DELTA 279-301 protein, in the presence of Pdx2, pH 8.0, 37°C | Plasmodium falciparum |
0.000779 | - |
wild-type protein, in the presence of Pdx2, pH 8.0, 37°C | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine | in the presence of Pdx2 | Plasmodium falciparum | pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? | |
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ | in the absence of Pdx2 | Plasmodium falciparum | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
pyridoxal 5-phosphate synthase | complex of Pdx1 and Pdx2 | Plasmodium falciparum |