Protein Variants | Comment | Organism |
---|---|---|
H170N | catalytically incompetent mutant of glutaminase subunit Pdx2 | Bacillus subtilis |
K149A | mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) does not form the imine adduct | Bacillus subtilis |
K149R | mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) retains the ability to form the imine adduct | Bacillus subtilis |
K81A | mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) does not form the imine adduct | Bacillus subtilis |
K81R | mutant of pyridoxal 5'-phosphate synthase subunit (Pdx1) does not form the imine adduct | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.126 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, wild-type enzyme | Bacillus subtilis | |
0.185 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, mutant enzyme K149R | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine | preference of D-ribose 5-phosphate compared to D-ribulose 5-phosphate. Characterization of a novel chromophoric reaction intermediate. The chromophoric group of this intermediate is appended to the epsilon-amino group of Lys81 and that the new residue has the composition C5H6O2, corresponding to the elimination of one equivalent of inorganic phosphate, one molecule of water one additional proton from the original protonated imine adduct | Bacillus subtilis | pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? | |
D-ribulose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine | preference of D-ribose 5-phosphate compared to D-ribulose 5-phosphate | Bacillus subtilis | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, mutant enzyme K149R | Bacillus subtilis | |
0.00067 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, free pyridoxal 5'-phosphate synthase subunit (Pdx1) | Bacillus subtilis | |
0.0007 | - |
D-ribulose 5-phosphate | pH 8.0, 37°C, free pyridoxal 5'-phosphate synthase subunit (Pdx1) | Bacillus subtilis | |
0.004 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, wild-type enzyme | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, mutant enzyme K149R | Bacillus subtilis | |
0.03 | - |
D-ribose 5-phosphate | pH 8.0, 37°C, wild-type enzyme | Bacillus subtilis |