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Literature summary for 4.3.3.1 extracted from

  • Kim, J.; Joo, J.; Kim, S.; Yoo, Y.
    Gene cloning and expression of a 3-ketovalidoxylamine C-N-lyase from Flavobacterium saccharophilum IFO 13984 (2011), Biotechnol. Bioprocess Eng., 16, 366-373.
No PubMed abstract available
Search for more literature for 4.3.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Flavobacterium saccharophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40878, calculated, x * 40000, SDS-PAGE Flavobacterium saccharophilum
40878
-
 x * 40878, calculated, x * 40000, SDS-PAGE Flavobacterium saccharophilum

Organism

Organism UniProt Comment Textmining
Flavobacterium saccharophilum DI109203
-
-
Flavobacterium saccharophilum IFO 13984 DI109203
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification putative N-terminal signal peptide, cleavage site is predicted between position 29 and 30 Flavobacterium saccharophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-ketovalidoxylamine A
-
 Flavobacterium saccharophilum (1S,2S,3R,6S)-6-amino-4-(hydroxymethyl)cyclohex-4-ene-1,2,3-triol + (5R,6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one i.e. valienamine ?
3-ketovalidoxylamine A
-
 Flavobacterium saccharophilum IFO 13984 (1S,2S,3R,6S)-6-amino-4-(hydroxymethyl)cyclohex-4-ene-1,2,3-triol + (5R,6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohex-2-en-1-one i.e. valienamine ?
3-ketovalidoxylamine A
-
 Flavobacterium saccharophilum (6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohexa-2,4-dien-1-one + (1R,2S,3S,4R,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol i.e. validamine ?
3-ketovalidoxylamine A
-
 Flavobacterium saccharophilum IFO 13984 (6R)-2,6-dihydroxy-5-(hydroxymethyl)cyclohexa-2,4-dien-1-one + (1R,2S,3S,4R,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol i.e. validamine ?

Subunits

Subunits Comment Organism
? x * 40878, calculated, x * 40000, SDS-PAGE Flavobacterium saccharophilum