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Literature summary for 4.3.3.1 extracted from

  • Zhang, J.F.; Zheng, Y.G.; Shen, Y.C.
    Purification and Characterization of 3-Ketovalidoxylamine A C-N Lyase Produced by Stenotrophomonas maltrophilia (2010), Appl. Biochem. Biotechnol., 162, 966-74.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2',2'',2'''-(1,2-ethanediyldinitrilo)tetraacetic acid
-
Stenotrophomonas maltophilia
4-nitroaniline inhibition at low concentration Stenotrophomonas maltophilia
Zn2+
-
Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
4-nitrophenyl-3-ketovalidamine
-
Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ promoting, reverses complete enzyme inhibition by EDTA Stenotrophomonas maltophilia
Zn2+ inhibitor Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
SDS-PAGE Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
367.5fold by column chromatography Stenotrophomonas maltophilia

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.94
-
after complete purification Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-3-ketovalidamine assay at pH 7.0 Stenotrophomonas maltophilia 4-nitroaniline + ?
-
?

Synonyms

Synonyms Comment Organism
3-ketovalidoxylamine A C-N lyase
-
Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
enzyme sensitive to heat Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10.5
-
Stenotrophomonas maltophilia

pI Value

Organism Comment pI Value Maximum pI Value
Stenotrophomonas maltophilia precast gel
-
7