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Literature summary for 4.3.3.1 extracted from

  • Takeuchi, M.; Asano, N.; Kameda, Y.; Matsui, K.
    Purification and properties of 3-ketovalidoxylamine A C-N lyase from Flavobacterium saccharophilum (1985), J. Biochem., 98, 1631-1638.
    View publication on PubMed
Search for more literature for 4.3.3.1

General Stability

General Stability Organism
glycerol stabilizes Flavobacterium saccharophilum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ at 1 mM, 37.4% inhibition Flavobacterium saccharophilum
Cu2+ at 1 mM, 13.5% inhibition Flavobacterium saccharophilum
EDTA i.e. ethylenediamine tetra acetic acid, at 1 mM, 37°C, for 20 min., 100% inhibition, Ca2+ reverses, not Mg2+ Flavobacterium saccharophilum
EGTA Ca2+ reverses; i.e. ethyleneglycol bis(beta-aminoethylether)-N,N'-tetraacetic acid, at 1 mM, 37°C, for 20 min., 100% inhibition Flavobacterium saccharophilum
La3+ at 1 mM, 97.4% inhibition Flavobacterium saccharophilum
Mn2+ at 1 mM, 92.8% inhibition Flavobacterium saccharophilum
additional information at 1 mM, 37°C, for 20 min., no inhibited by p-chloromercuribenzoate and Mg2+, insignificant inhibition by Hg2+ Flavobacterium saccharophilum
Sr2+ at 1 mM, 69.0% inhibition Flavobacterium saccharophilum
Zn2+ at 1 mM, 55.5% inhibition Flavobacterium saccharophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 N-4-nitrophenyl-3-ketovalidamine pH 8, 37°C Flavobacterium saccharophilum
0.5
-
 p-nitrophenyl-alpha-D-3-ketoglucoside pH 7, 37°C Flavobacterium saccharophilum
2.5
-
 N-4-Nitrophenyl-3-ketovalienamine pH 7, 37°C Flavobacterium saccharophilum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Flavobacterium saccharophilum 16020
-
soluble
-
 Flavobacterium saccharophilum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Flavobacterium saccharophilum
Ca2+ positive cooperative interaction, maximal activation at 0.003 mM, kinetics Flavobacterium saccharophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration Flavobacterium saccharophilum

Organism

Organism UniProt Comment Textmining
Flavobacterium saccharophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from soluble fraction, by ammonium sulfate fractionation, column chromatography on CM cellulose and gel filtration on Sephacryl S-200 Flavobacterium saccharophilum

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.73
-
-
 Flavobacterium saccharophilum

Storage Stability

Storage Stability Organism
4°C, in 0.05 M phosphate buffer, pH 7, 60% loss of activity within 2 weeks, 2 weeks stable in the presence of 10% glycerol Flavobacterium saccharophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has C-O-lyase activity Flavobacterium saccharophilum ?
-
 ?
additional information no substrates are O-4-nitrophenyl-beta-D-3-ketoglucoside, N-4-nitrophenylvalidamine, N-4-nitrophenyl-1-epi-3-ketovalidamine, O-4-nitrophenyl-alpha-D-glucoside Flavobacterium saccharophilum ?
-
 ?
additional information no substrates are N-4-nitrophenylvalienamine, O-4-nitrophenyl-beta-D-glucoside Flavobacterium saccharophilum ?
-
 ?
N-4-nitrophenyl-3-ketovalidamine synthetic model compound Flavobacterium saccharophilum 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one
-
 ?
N-4-nitrophenyl-3-ketovalienamine synthetic model compound Flavobacterium saccharophilum 4-nitroaniline + ?
-
 ?
O-4-nitrophenyl-alpha-D-3-ketoglucoside synthetic model compound Flavobacterium saccharophilum 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose
-
 ?
O-4-nitrophenyl-alpha-D-3-ketoglucoside best substrate, C-O-lyase reaction Flavobacterium saccharophilum 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Flavobacterium saccharophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Flavobacterium saccharophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Flavobacterium saccharophilum

pH Range

pH Minimum pH Maximum Comment Organism
5 11 no activity below pH 5 and above pH 11 Flavobacterium saccharophilum