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Literature summary for 4.3.2.9 extracted from
- Defining the cytosolic pathway of glutathione degradation in Arabidopsis thaliana: role of the ChaC/GCG family of gamma-glutamyl cyclotransferases as glutathione-degrading enzymes and AtLAP1 as the Cys-Gly peptidase (2015), Biochem. J., 468, 73-85.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 pLys2(DE3) cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
glutathione | isoform GGCT2;2, at pH 8.0 and 37°C | Arabidopsis thaliana |  |
| 4.96 | - |
glutathione | isoform GGCT2;3, at pH 8.0 and 37°C | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-L-glutamyl-L-alanine | low activity compared to glutathione | Arabidopsis thaliana | 5-oxoproline + L-alanine | - |
? | |
| glutathione | - |
Arabidopsis thaliana | 5-oxoproline + L-Cys-Gly | - |
? | |
| additional information | no significant activity towards gamma-Glu-Cys | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-glutamyl cyclotransferases | - |
Arabidopsis thaliana |
| GGCT | - |
Arabidopsis thaliana |
| GGCT2;2 | isoform | Arabidopsis thaliana |
| GGCT2;3 | isoform | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.113 | - |
glutathione | isoform GGCT2;3, at pH 8.0 and 37°C | Arabidopsis thaliana | |
| 0.64 | - |
glutathione | isoform GGCT2;2, at pH 8.0 and 37°C | Arabidopsis thaliana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
glutathione | isoform GGCT2;3, at pH 8.0 and 37°C | Arabidopsis thaliana | |
| 0.378 | - |
glutathione | isoform GGCT2;2, at pH 8.0 and 37°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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