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Literature summary for 4.3.2.5 extracted from

  • Kolhekar, A.S.; Bell, J.; Shiozaki, E.N.; Jin, L.; Keutmann, H.T.; Hand, T.A.; Mains, R.E.; Eipper, B.A.
    Essential features of the catalytic core of peptidyl-a-hydroxyglycine alpha-amidating Lyase (2002), Biochemistry, 41, 12384-12394.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Chinese hamster ovary cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D590N no secretion Rattus norvegicus
D599N no secretion Rattus norvegicus
D652N no secretion Rattus norvegicus
D705N much less secretion and less active than wild-type Rattus norvegicus
E707Q much less secretion than wild-type Rattus norvegicus
H532A no secretion Rattus norvegicus
H585A less active than wild-type Rattus norvegicus
H603A no secretion Rattus norvegicus
H690A less active than wild-type Rattus norvegicus
H697A no changes in secretion an activity Rattus norvegicus
H786A less active than wild-type Rattus norvegicus
H817A no changes in secretion an activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Rattus norvegicus
EDTA addition of 0.5-1 M reduces activity to 50%, Ca2+, Mn2+, Co2+ or Ni2+ restore activity Rattus norvegicus
EGTA addition of 0.5-1 M reduces activity to 50%, Ca2+, Mn2+, Co2+ or Ni2+ restore activity Rattus norvegicus
HCl 0.5 M guanidinium HCl reduces activity to 28% Rattus norvegicus
o-phenanthroline
-
Rattus norvegicus
Urea addition of 1 M urea reduces activity to 58% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0102
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, E707Q Rattus norvegicus
0.0139
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, recombinant wild-type Rattus norvegicus
0.0156
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, H690A Rattus norvegicus
0.0214
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, D705N Rattus norvegicus
0.0231
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, H585A Rattus norvegicus
0.0985
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5, H786A Rattus norvegicus
0.33
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 0.7 mol per mol enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-acetyl-Tyr-Val-alpha-hydroxyglycine
-
Rattus norvegicus Nalpha-acetyl-Tyr-Val-NH2 + glyoxylate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
380
-
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 5.5 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.5
-
Rattus norvegicus