Cloned (Comment) | Organism |
---|---|
expression in Chinese hamster ovary cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D590N | no secretion | Rattus norvegicus |
D599N | no secretion | Rattus norvegicus |
D652N | no secretion | Rattus norvegicus |
D705N | much less secretion and less active than wild-type | Rattus norvegicus |
E707Q | much less secretion than wild-type | Rattus norvegicus |
H532A | no secretion | Rattus norvegicus |
H585A | less active than wild-type | Rattus norvegicus |
H603A | no secretion | Rattus norvegicus |
H690A | less active than wild-type | Rattus norvegicus |
H697A | no changes in secretion an activity | Rattus norvegicus |
H786A | less active than wild-type | Rattus norvegicus |
H817A | no changes in secretion an activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rattus norvegicus | |
EDTA | addition of 0.5-1 M reduces activity to 50%, Ca2+, Mn2+, Co2+ or Ni2+ restore activity | Rattus norvegicus | |
EGTA | addition of 0.5-1 M reduces activity to 50%, Ca2+, Mn2+, Co2+ or Ni2+ restore activity | Rattus norvegicus | |
HCl | 0.5 M guanidinium HCl reduces activity to 28% | Rattus norvegicus | |
o-phenanthroline | - |
Rattus norvegicus | |
Urea | addition of 1 M urea reduces activity to 58% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5, E707Q | Rattus norvegicus | |
0.0139 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5, recombinant wild-type | Rattus norvegicus | |
0.0156 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5, H690A | Rattus norvegicus | |
0.0214 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5, D705N | Rattus norvegicus | |
0.0231 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5, H585A | Rattus norvegicus | |
0.0985 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5, H786A | Rattus norvegicus | |
0.33 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 0.7 mol per mol enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-acetyl-Tyr-Val-alpha-hydroxyglycine | - |
Rattus norvegicus | Nalpha-acetyl-Tyr-Val-NH2 + glyoxylate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
380 | - |
Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine | 37°C, pH 5.5 | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | - |
Rattus norvegicus |