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Literature summary for 4.3.2.5 extracted from

  • Eipper, B.A.; Perkins, S.N.; Husten, E.J.; Johnson, R.C.; Keutmann, H.T.; Mains, R.E.
    Peptidyl-alpha-hydroxyglycine alpha-amidating lyase. Purification, characterization, and expressio (1991), J. Biol. Chem., 266, 7827-7833.
    View publication on PubMed
Search for more literature for 4.3.2.5

Inhibitors

Inhibitors Comment Organism Structure
EDTA divalent metals restore activity Bos taurus
K2SO4 150 mM Bos taurus
KCl 150 mM Bos taurus
additional information relatively resistant to thiol reagents and urea Bos taurus
NaCl 150 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 PAL domain, conversion of the 50000 MW form into a 43000 MW form occurs during gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
hypophysis neurointermediate Bos taurus
-
hypophysis granules Bos taurus
-
pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-acetyl-Tyr-Val-alpha-hydroxyglycine
-
 Bos taurus Nalpha-acetyl-Tyr-Val-NH2 + glyoxylate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
220
-
 Nalpha-Acetyl-Tyr-Val-alpha-hydroxyglycine 37°C, pH 7 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bos taurus