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Literature summary for 4.3.2.2 extracted from

  • Segall, M.L.; Colman, R.F.
    Gln212, Asn270, and Arg301 are critical for catalysis by adenylosuccinate lyase from Bacillus subtilis (2004), Biochemistry, 43, 7391-7402.
    View publication on PubMed
Search for more literature for 4.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as His tag enzymes Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
N270D mutant with impaired activity Bacillus subtilis
N270L mutant with impaired activity Bacillus subtilis
Q212E mutant with impaired activity Bacillus subtilis
Q212M mutant with impaired activity Bacillus subtilis
R301K mutant with impaired activity Bacillus subtilis
R301Q mutant with impaired activity Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 adenylosuccinate wild type, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
0.0166
-
 adenylsuccinate mutant Q212E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Bacillus subtilis
50000
-
 4 * 50000, SDS-PAGE Bacillus subtilis
152000
-
 mutant N270L, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
153000
-
 wild type, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
159000
-
 mutant Q212E, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
166000
-
 mutant N270D, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
173000
-
 mutant R301K, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
174000
-
 mutant Q212M, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
200000
-
 SDS-PAGE Bacillus subtilis
219000
-
 mutant R301Q, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Bacillus subtilis
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
adenylosuccinate Bacillus subtilis
-
 AMP + fumarate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Bacillus subtilis 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
adenylosuccinate
-
 Bacillus subtilis AMP + fumarate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Bacillus subtilis
homotetramer 4 * 50000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
ASL
-
 Bacillus subtilis