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Literature summary for 4.3.2.10 extracted from

  • Klem, T.J.; Davisson, V.J.
    Imidazole glycerol phosphate synthase the glutamine amidotransferase in histidine biosynthesis (1993), Biochemistry, 32, 5177-5186 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate the catalytic turnover of glutamine can be increased up to 37fold by the addition of either the product D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate or the biosynthetic precursor N1-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide Escherichia coli
N1-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide the catalytic turnover of glutamine can be increased up to 37fold by the addition of either the product D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate or the biosynthetic precursor N1-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli FB1 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 8.0, 30°C, cosubstrate: L-glutamine Escherichia coli
0.023
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 8.0, 30°C, cosubstrate: NH4+ Escherichia coli
0.24
-
L-glutamine pH 8.0, 30°C Escherichia coli
291
-
NH4+ pH 8.0, 30°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
1:1 dimeric complex of HisH and HisF proteins, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Escherichia coli the enzyme is involved in histidine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Escherichia coli K12 the enzyme is involved in histidine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme is involved in histidine biosynthesis Escherichia coli 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine glutamine is a more efficient substrate relative to ammonium ion by a factor of 1000 Escherichia coli 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme is involved in histidine biosynthesis Escherichia coli K12 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine glutamine is a more efficient substrate relative to ammonium ion by a factor of 1000 Escherichia coli K12 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+ glutamine is a more efficient substrate relative to ammonium ion by a factor of 1000 Escherichia coli 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+ glutamine is a more efficient substrate relative to ammonium ion by a factor of 1000 Escherichia coli K12 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
?

Subunits

Subunits Comment Organism
dimer 1 * 21653 (subunit HisH) + 1 * 28454 (subunit HisF), calculated from sequence, HisH and HisF proteins form a stable 1:1 dimeric complex that constitutes the IGP synthase holoenzyme Escherichia coli

Synonyms

Synonyms Comment Organism
hisFH
-
Escherichia coli
IGP synthase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 8.0, 30°C, cosubstrate: NH4+ Escherichia coli
8.5
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 8.0, 30°C, cosubstrate: L-glutamine Escherichia coli
8.8
-
NH4+ pH 8.0, 30°C Escherichia coli
9.1
-
L-glutamine pH 8.0, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 activity with glutamine Escherichia coli
8 9 activity with NH4+ Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 the pH optimum with glutamine is broad over the range of values from 6.0 to 8.0 but diminishes to 85% at pH 8.5 and 22% at pH 9.5 Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme is involved in histidine biosynthesis Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
NH4+ pH 8.0, 30°C Escherichia coli
37.9
-
L-glutamine pH 8.0, 30°C Escherichia coli
265.2
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 8.0, 30°C, cosubstrate: NH4+ Escherichia coli
5667
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 8.0, 30°C, cosubstrate: L-glutamine Escherichia coli