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Literature summary for 4.3.2.1 extracted from
- Argininosuccinate lyase: purification and characterization from human liver (1981), Biochemistry, 20, 2056-2060.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing does not appreciably alter the activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
argininosuccinate | - |
Homo sapiens |  |
| 3 | - |
Arg | - |
Homo sapiens | |
| 5.3 | - |
fumarate | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
4 * 49000, SDS-PAGE | Homo sapiens |
| 187000 | - |
equilibrium sedimentation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
formation of argininosuccinate | Homo sapiens |
| 10.3 | - |
cleavage of argininosuccinate | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen in 0.1 M potassium phosphate, pH 7.0, stable | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| argininosuccinate | - |
Homo sapiens | fumarate + L-Arg | - |
r | |
| fumarate + L-Arg | - |
Homo sapiens | argininosuccinate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 49000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.2 | formation of argininosuccinate | Homo sapiens |
| 7.5 | - |
formation of argininosuccinate | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
at 4°C and at 25°C, 8 h, stable | Homo sapiens |
| 8.2 | - |
25°C, about 40% loss of activity after 8 h | Homo sapiens |
| 8.2 | - |
4°C, about 60% loss of activity after 4 h | Homo sapiens |
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Release 2023.1 (January 2023)
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