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Literature summary for 4.3.1.B2 extracted from

  • Myers, R.S.; Jensen, J.R.; Deras, I.L.; Smith, J.L.; Davisson, V.J.
    Substrate-induced changes in the ammonia channel for imidazole glycerol phosphate synthase (2003), Biochemistry, 42, 7013-7022.
    View publication on PubMed
Search for more literature for 4.3.1.B2

Protein Variants

Protein Variants Comment Organism
K258A ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 43:1 (wild-type ratio is 1:1), 2600fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 385fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 1055fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
K258R ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 3:1 (wild-type ratio is 1:1), 20fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 125fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 45fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
K360R ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 1:1 (identical to wild-type ratio), 1090fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 3.5fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 6.4fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
R239A ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 122:1 (wild-type ratio is 1:1), 860fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 5.4fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 3450fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
R239H ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 154:1 (wild-type ratio is 1:1), 2400fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 5.2fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 1360fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
R239K ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 40:1 (wild-type ratio is 1:1), 218fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 3.9fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 540fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
R360A ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 3:1 (wild-type ratio is 1:1), 9.2fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 1.4fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 15fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
albizziin mixed type inhibition versus N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, competitive inhibition versus L-glutamine Saccharomyces cerevisiae
N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide competitive versus N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mixed type inhibition versus L-glutamine Saccharomyces cerevisiae
N1-[(5'-phosphoribitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide competitive versus N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K Saccharomyces cerevisiae
0.0018
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A Saccharomyces cerevisiae
0.002
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R Saccharomyces cerevisiae
0.0023
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R Saccharomyces cerevisiae
0.003
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A Saccharomyces cerevisiae
0.005
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
0.008
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H Saccharomyces cerevisiae
0.046
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R Saccharomyces cerevisiae
0.053
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A Saccharomyces cerevisiae
0.055
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
0.065
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A Saccharomyces cerevisiae
0.072
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R Saccharomyces cerevisiae
0.075
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H Saccharomyces cerevisiae
0.08
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K Saccharomyces cerevisiae
0.098
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A Saccharomyces cerevisiae
0.139
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A Saccharomyces cerevisiae
1.3
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K Saccharomyces cerevisiae
1.8
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme Saccharomyces cerevisiae
1.9
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R Saccharomyces cerevisiae
1.9
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R Saccharomyces cerevisiae
1.96
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A Saccharomyces cerevisiae
2.1
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H Saccharomyces cerevisiae
6.5
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A Saccharomyces cerevisiae
6.5
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P33734
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
additional information the glutaminase activity of the enzyme is stimulated 4900fold in the presence of the acceptor substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae ?
-
 ?
NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Synonyms

Synonyms Comment Organism
IGP synthase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A Saccharomyces cerevisiae
0.045
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A Saccharomyces cerevisiae
0.126
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R Saccharomyces cerevisiae
0.15
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A Saccharomyces cerevisiae
0.16
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R Saccharomyces cerevisiae
0.21
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H Saccharomyces cerevisiae
0.24
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A Saccharomyces cerevisiae
0.29
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R Saccharomyces cerevisiae
0.3
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K Saccharomyces cerevisiae
0.31
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R Saccharomyces cerevisiae
0.49
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A Saccharomyces cerevisiae
0.7
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A Saccharomyces cerevisiae
0.845
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
1.13
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R Saccharomyces cerevisiae
3.9
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H Saccharomyces cerevisiae
4.3
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A Saccharomyces cerevisiae
5
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R Saccharomyces cerevisiae
5.4
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
5.4
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A Saccharomyces cerevisiae
6
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H Saccharomyces cerevisiae
6.9
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A Saccharomyces cerevisiae
6.9
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme Saccharomyces cerevisiae
8.7
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K Saccharomyces cerevisiae
9
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00067
-
 N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide pH 7.0, 30°C, substrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae
0.0016
-
 N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide pH 7.0, 30°C, substrate: L-glutamine, uncompetitive inhibition constant Saccharomyces cerevisiae
0.003
-
 N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide pH 7.0, 30°C, substrate: L-glutamine, competitive inhibition constant Saccharomyces cerevisiae
0.004
-
 N1-[(5'-phosphoribitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide pH 7.0, 30°C, N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae
0.38
-
 albizziin pH 7.0, 30°C, substrate: L-glutamine Saccharomyces cerevisiae
0.6
-
 albizziin pH 7.0, 30°C, competitive inhibition constant, substrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae
1.6
-
 albizziin pH 7.0, 30°C, uncompetitive inhibition constant, substrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0011
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A Saccharomyces cerevisiae
0.0028
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H Saccharomyces cerevisiae
0.0036
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A Saccharomyces cerevisiae
0.007
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K Saccharomyces cerevisiae
0.039
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A Saccharomyces cerevisiae
0.084
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R Saccharomyces cerevisiae
0.12
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R Saccharomyces cerevisiae
0.247
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A Saccharomyces cerevisiae
0.46
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A Saccharomyces cerevisiae
0.5
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H Saccharomyces cerevisiae
0.59
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R Saccharomyces cerevisiae
1.4
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A Saccharomyces cerevisiae
2.8
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A Saccharomyces cerevisiae
2.9
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H Saccharomyces cerevisiae
3.8
-
 L-glutamine pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme Saccharomyces cerevisiae
3.8
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K Saccharomyces cerevisiae
4.3
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R Saccharomyces cerevisiae
5.5
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K Saccharomyces cerevisiae
11
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A Saccharomyces cerevisiae
15
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
60
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R Saccharomyces cerevisiae
110
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R Saccharomyces cerevisiae
130
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A Saccharomyces cerevisiae
1200
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae