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Literature summary for 4.3.1.30 extracted from

  • Ruszczycky, M.W.; Choi, S.H.; Liu, H.W.
    EPR-kinetic isotope effect study of the mechanism of radical-mediated dehydrogenation of an alcohol by the radical SAM enzyme DesII (2013), Proc. Natl. Acad. Sci. USA, 110, 2088-2093.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
TDP-D-quinovose substrate inhibition Streptomyces venezuelae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
S-adenosyl-L-methionine pH 8.0, temperature not specified in the publication Streptomyces venezuelae
0.2
-
TDP-D-quinovose pH 8.0, temperature not specified in the publication Streptomyces venezuelae

Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae Q9ZGH1
-
-

Reaction

Reaction Comment Organism Reaction ID
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor = dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor DesII must be in the correct protonation state to form the initial alpha-hydroxyalkyl radical and that it is subsequently deprotonated either before or concerted with its oxidation Streptomyces venezuelae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TDP-D-quinovose + S-adenosyl-L-methionine
-
Streptomyces venezuelae ?
-
?

Synonyms

Synonyms Comment Organism
DesII
-
Streptomyces venezuelae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptomyces venezuelae