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Literature summary for 4.3.1.3 extracted from

  • Hellio, C.; Veron, B.; Le Gal, Y.
    Amino acid utilization by Chlamydomonas reinhardtii: Specific study of histidine (2004), Plant Physiol. Biochem., 42, 257-264.
    View publication on PubMed

General Stability

General Stability Organism
rapid decrease of activity in presence of mercaptoethanol or cysteine, 56% of initial activity after 1 d, 20% of initial activity after 2 d. In presence of DTT, the enzyme remains stable for several days Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
Co3+ 0.1 mM, 95% inhibition Chlamydomonas reinhardtii
Cu2+ 0.1 mM, 70% inhibition Chlamydomonas reinhardtii
cyanide 50 mM histidinol phosphate, 5 mM L-histidine and 5 mM Mn2+ protects Chlamydomonas reinhardtii
Fe2+ 0.1 mM, 60% inhibition Chlamydomonas reinhardtii
Fe3+ 0.1 mM, 60% inhibition Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the enzyme does not exhibit classical Michaelis-Menten kinetics Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.1 mM, activation to 124.5% of control Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38100
-
4 * 38100, SDS-PAGE Chlamydomonas reinhardtii
152400
-
gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
CCAP 11/32A
-
Chlamydomonas reinhardtii CCAP 11/32A
-
CCAP 11/32A
-

Purification (Commentary)

Purification (Comment) Organism
-
Chlamydomonas reinhardtii

Source Tissue

Source Tissue Comment Organism Textmining
additional information grown axenically at 18°C under continous illumination. Cells grown on ammonium as the sole nitrogen source contain no histidase activity, cells grown on histidine, on histidine and glycine, or on histidine and aspartate contain histidase and exhibited similar histidase activities. When the cells are grown on histidine and ammonium, or on histidine and succinate, or on histidine and glutamate, the production of histidase is slightly delayed and reaches a lower level than when cells were grown on histidine as the sole source of nitrogen. No histidase activity was detected when C. reinhardtii was grown in the presence of acetate Chlamydomonas reinhardtii
-

Subunits

Subunits Comment Organism
tetramer 4 * 38100, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.2
-
Chlamydomonas reinhardtii