Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Pseudomonas sp. | |
dithiothreitol | activates | Pseudomonas sp. | |
GSH | activates | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
biguanide | - |
Pseudomonas sp. | |
bisulfite | - |
Pseudomonas putida | |
bisulfite | sodiumbisulfite | Pseudomonas sp. | |
CN- | KCN | Pseudomonas putida | |
CN- | KCN | Pseudomonas sp. | |
EDTA | - |
Pseudomonas putida | |
EDTA | - |
Pseudomonas sp. | |
hydroxylamine | - |
Pseudomonas sp. | |
L-cysteine | - |
Pseudomonas putida | |
L-cysteine | - |
Pseudomonas sp. | |
L-tyrosine | heat-purified enzyme, at low concentrations, pH 7-8 | Pseudomonas sp. | |
NaBH4 | - |
Pseudomonas sp. | |
p-hydroxyphenylpyruvate | heat-purified enzyme, at low concentrations, pH 7-8 | Pseudomonas sp. | |
p-mercuribenzoate | inhibits mercaptoethanol-activated enzyme | Pseudomonas sp. | |
phenylhydrazine | - |
Pseudomonas sp. | |
Sodium diphosphate | - |
Pseudomonas sp. | |
Urocanate | - |
Pseudomonas aeruginosa | |
Urocanate | heat-purified enzyme, at low concentrations, pH 7-8 | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
L-histidine | 25°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | enhances activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
- |
Pseudomonas aeruginosa |
210000 | - |
sedimentation equilibrium | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas sp. | - |
ATCC 11,299b | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
additional information | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
2°C, 0.1 M potassium phosphate, pH 7.2, 0.1 mM MnCl2 saturated with chloroform, stable for 9 months without loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidine | natural substrate | Pseudomonas aeruginosa | urocanate + NH3 | - |
? | |
L-histidine | natural substrate | Pseudomonas sp. | urocanate + NH3 | - |
? | |
L-histidine | natural substrate | Pseudomonas putida | urocanate + NH3 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.1 | 9.9 | 50% of maximal activity at pH 8.1 and pH 9.9 | Pseudomonas sp. |