Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.29 extracted from

  • Phillips, R.S.; Ting, S.C.; Tetsadjio, A.G.; Anderson, K.L.; Friez, K.M.; Miller, K.A.; Hoover, T.R.
    Properties and mechanism of D-glucosaminate-6-phosphate ammonia-lyase An aminotransferase family enzyme with D-amino acid specificity (2018), Biochim. Biophys. Acta, 1866, 799-805 .
    View publication on PubMed
Search for more literature for 4.3.1.29

Inhibitors

Inhibitors Comment Organism Structure
alpha-glycerophosphate weak competitive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
additional information no significant inhibition with D-serine at concentrations up to 200 mM Salmonella enterica subsp. enterica serovar Typhimurium
phosphate weak competitive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 2-amino-2-deoxy-D-gluconate 6-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
KCl modest (about 30%) activation at 0.05 M, strongly inhibition (more than 50%) at higher concentrations (0.5 M) Salmonella enterica subsp. enterica serovar Typhimurium
NaCl modest (about 30%) activation at 0.05 M, strongly inhibition (more than 50%) at higher concentrations (0.5 M) Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-2-deoxy-D-gluconate 6-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium D0ZLR3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxy-D-gluconate 6-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
-
 ?
2-amino-2-deoxy-D-gluconate 6-phosphate the enzyme is highly efficient and highly specific for 2-amino-2-deoxy-D-gluconate 6-phosphate. A mechanism is proposed that requires a second catalytic base located on the D-face of the substrate-pyridoxal-5'-phosphate complex. Deprotonation is followed by elimination of water to give an aminoacrylate intermediate, which tautomerizes and undergoes hydrolysis to give the product, 2-dehydro-3-deoxygluconate-6-phosphate Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39323, calculated from sequence Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
DGL
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.4
-
 2-amino-2-deoxy-D-gluconate 6-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a second catalytic base located on the D-face of the substrate-pyridoxal 5'-phosphate complex. Deprotonation is followed by elimination of water to give an aminoacrylate intermediate, which tautomerizes and undergoes hydrolysis to give the product, 2-dehydro-3-deoxygluconate-6-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
32
-
 alpha-glycerophosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
38
-
 phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
196
-
 2-amino-2-deoxy-D-gluconate 6-phosphate pH 8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium