Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Delftia sp. HT23 |
Protein Variants | Comment | Organism |
---|---|---|
K43A | the mutant enzyme shows no detectable activity | Delftia sp. HT23 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the enzyme is modestly inhibited by EDTA (27% inhibition at 1 mM) | Delftia sp. HT23 | |
hydroxylamine | the enzyme is strongly inhibited by hydroxylamine (91.2% inhibition at 1 mM) | Delftia sp. HT23 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
D-serine | at pH 8.5 and 50°C | Delftia sp. HT23 | |
0.16 | - |
L-erythro-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
0.42 | - |
D-threo-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
6.16 | - |
L-threo-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
38.7 | - |
L-serine | at pH 8.5 and 50°C | Delftia sp. HT23 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activator | Delftia sp. HT23 | |
Co2+ | the recombinant enzyme is highly activated by Co2+ | Delftia sp. HT23 | |
Fe2+ | activator | Delftia sp. HT23 | |
Mn2+ | the recombinant enzyme is highly activated by Mn2+ | Delftia sp. HT23 | |
additional information | no activity is detected when Sn2+ or Cu2+ is added | Delftia sp. HT23 | |
Ni2+ | the recombinant enzyme is highly activated by Ni2+ | Delftia sp. HT23 | |
Zn2+ | activator | Delftia sp. HT23 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Delftia sp. HT23 |
40300 | - |
calculated from amino acid sequence | Delftia sp. HT23 |
40900 | - |
calculated from the deduced amino acid sequence of the recombinant enzyme | Delftia sp. HT23 |
41000 | - |
1 * 41000, SDS-PAGE | Delftia sp. HT23 |
41600 | - |
MALDI-TOF mass spectrometry | Delftia sp. HT23 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia sp. HT23 | B2DFG5 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiPrep Q column chromatography, HiTrap phenyl column chromatography, Superdex-200 gel filtration, Resource Q column chromatography, and HiTrap butyl column chromatography | Delftia sp. HT23 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
cell extract, at 50°C, pH 8.5 | Delftia sp. HT23 |
21.3 | - |
after 115.8fold purification, at 50°C, pH 8.5 | Delftia sp. HT23 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine | poor substrate | Delftia sp. HT23 | ? | - |
? | |
D-threo-3-hydroxyaspartate | - |
Delftia sp. HT23 | oxaloacetate + NH3 | - |
? | |
L-erythro-3-hydroxyaspartate | - |
Delftia sp. HT23 | ? | - |
? | |
L-serine | poor substrate | Delftia sp. HT23 | ? | - |
? | |
L-threo-3-hydroxyaspartate | - |
Delftia sp. HT23 | oxaloacetate + NH3 | - |
? | |
L-threo-3-hydroxyaspartate | - |
Delftia sp. HT23 | ? | - |
? | |
additional information | D-erythro-3-hydroxyaspartate is not a substrate | Delftia sp. HT23 | ? | - |
? | |
additional information | the purified enzyme shows no alanine racemase activity | Delftia sp. HT23 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Delftia sp. HT23 |
Synonyms | Comment | Organism |
---|---|---|
D-THA DH | - |
Delftia sp. HT23 |
D-threo-3-hydroxyaspartate ammonia-lyase | - |
Delftia sp. HT23 |
D-threo-3-hydroxyaspartate dehydratase | - |
Delftia sp. HT23 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Delftia sp. HT23 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-serine | at pH 8.5 and 50°C | Delftia sp. HT23 | |
3.03 | - |
L-threo-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
8.68 | - |
D-serine | at pH 8.5 and 50°C | Delftia sp. HT23 | |
8.68 | - |
L-erythro-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
10.93 | - |
D-threo-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Delftia sp. HT23 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Delftia sp. HT23 |
Organism | Comment | Expression |
---|---|---|
Delftia sp. HT23 | the enzyme is not induced by D-serine, D-threonine, D-aspartate, or peptone | additional information |
Delftia sp. HT23 | the enzyme is induced by 3-hydroxyaspartate in the medium | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
L-serine | at pH 8.5 and 50°C | Delftia sp. HT23 | |
0.49 | - |
L-threo-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
5.91 | - |
D-serine | at pH 8.5 and 50°C | Delftia sp. HT23 | |
25.96 | - |
D-threo-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 | |
54.25 | - |
L-erythro-3-hydroxyaspartate | at pH 8.5 and 50°C | Delftia sp. HT23 |