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Literature summary for 4.3.1.25 extracted from
- Cloning, expression, site-directed mutagenesis and immunolocalization of phenylalanine ammonia-lyase in Bambusa oldhamii (2010), Phytochemistry, 71, 1999-2009.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bambusa oldhamii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.097 | - |
L-Tyr | pH 8.5, 37°C | Bambusa oldhamii |  |
| 2.07 | - |
L-Phe | pH 8.5, 37°C | Bambusa oldhamii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75711 | - |
4 * 75711, calculated | Bambusa oldhamii |
| 290000 | - |
gel filtration | Bambusa oldhamii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bambusa oldhamii | D5KS97 | isoform PAL4 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shell | - |
Bambusa oldhamii | - |
| shoot | in shoot and branch shoot, mainly loclaized to sclerenchymal cells | Bambusa oldhamii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe | - |
Bambusa oldhamii | (E)-cinnamate + NH3 | - |
? | |
| L-Tyr | - |
Bambusa oldhamii | p-coumarate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 75711, calculated | Bambusa oldhamii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAL4 | - |
Bambusa oldhamii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bambusa oldhamii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
L-Tyr | pH 8.5, 37°C | Bambusa oldhamii | |
| 16.32 | - |
L-Phe | pH 8.5, 37°C | Bambusa oldhamii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bambusa oldhamii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.56 | - |
L-Tyr | pH 8.5, 37°C | Bambusa oldhamii | |
| 7.88 | - |
L-Phe | pH 8.5, 37°C | Bambusa oldhamii | |
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