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Literature summary for 4.3.1.24 extracted from

  • Jun, S.Y.; Sattler, S.A.; Cortez, G.S.; Vermerris, W.; Sattler, S.E.; Kang, C.
    Biochemical and structural analysis of substrate specificity of a phenylalanine ammonia-lyase (2018), Plant Physiol., 176, 1452-1468 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5 2 L-phenylalanine pH 8.0, 30°C, mutant enzyme Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75600
-
-
Sorghum bicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine Sorghum bicolor enzyme of the phenylpropanoid pathway trans-cinnamate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor C5XXT9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
Sorghum bicolor trans-cinnamate + NH3
-
?
L-phenylalanine enzyme of the phenylpropanoid pathway Sorghum bicolor trans-cinnamate + NH3
-
?

Synonyms

Synonyms Comment Organism
Sb04g026520
-
Sorghum bicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sorghum bicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
L-phenylalanine pH 8.0, 30°C Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Sorghum bicolor

Cofactor

Cofactor Comment Organism Structure
3,5-dihydro-5-methylidene-4H-imidazol-4-one prosthetic group Sorghum bicolor

General Information

General Information Comment Organism
metabolism enzyme of the phenylpropanoid pathway Sorghum bicolor

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.45
-
L-phenylalanine pH 8.0, 30°C, mutant enzyme Sorghum bicolor