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Literature summary for 4.3.1.24 extracted from
- Cloning, expression and characterization of phenylalanine ammonia-lyase from Rhodotorula glutinis (2013), Biotechnol. Lett., 35, 751-756.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Rhodotorula glutinis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | strong inhibition | Rhodotorula glutinis |  |
| Cu2+ | 60% inhibition | Rhodotorula glutinis | |
| Fe3+ | 60% inhibition | Rhodotorula glutinis | |
| Mn2+ | strong inhibition | Rhodotorula glutinis | |
| Zn2+ | 60% inhibition | Rhodotorula glutinis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.34 | - |
L-phenylalanine | pH 8, 40°C | Rhodotorula glutinis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75500 | - |
calculated from cDNA | Rhodotorula glutinis |
| 309000 | - |
gel filtration | Rhodotorula glutinis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation, anion-exchange, and gel filtration chromatography | Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine | - |
Rhodotorula glutinis | (E)-cinnamate + NH3 | - |
? | |
| additional information | enzyme shows a higher affinity towards substrate L-tyrosine compared to L-phenylalanine | Rhodotorula glutinis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | SDS-PAGE, 4 * 75500 | Rhodotorula glutinis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RgPAL | - |
Rhodotorula glutinis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Rhodotorula glutinis |
| 50 | - |
- |
Rhodotorula glutinis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
80% activity after 1h | Rhodotorula glutinis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.7 | - |
L-phenylalanine | pH 8, 40°C | Rhodotorula glutinis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rhodotorula glutinis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | optimal activity | Rhodotorula glutinis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Rhodotorula glutinis |
| 11 | - |
enzyme retains 85% activity | Rhodotorula glutinis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19200 | - |
L-phenylalanine | pH 8, 40°C | Rhodotorula glutinis | |
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