Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.24 extracted from

  • Bartsch, S.; Bornscheuer, U.
    Mutational analysis of phenylalanine ammonia lyase to improve reactions rates for various substrates (2010), Protein Eng. Des. Sel., 23, 929-933.
    View publication on PubMed
Search for more literature for 4.3.1.24

Protein Variants

Protein Variants Comment Organism
F137I increased activity in the amination of p-nitro-cinnamic acid Petroselinum crispum
F137T increased activity in the amination of p-nitro-cinnamic acid Petroselinum crispum
F137V increased activity in the amination of p-nitro-cinnamic acid Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 (2E)-4-methyl-cinnamic acid mutant F137V, pH 10, 30°C Petroselinum crispum
0.1
-
 (2E)-4-formyl-cinnamic acid mutant F137V, pH 10, 30°C Petroselinum crispum
0.2
-
 (E)-cinnamate mutant F137V, pH 10, 30°C Petroselinum crispum
0.2
-
 (E)-cinnamate wild-type, pH 10, 30°C Petroselinum crispum
0.3
-
 (2E)-4-fluoro-cinnamic acid wild-type, pH 10, 30°C Petroselinum crispum
0.4
-
 4-fluoro-L-phenylalanine mutant F137V, pH 10, 30°C Petroselinum crispum
0.4
-
 L-tyrosine wild-type, pH 10, 30°C Petroselinum crispum
0.4
-
 4-fluoro-L-phenylalanine wild-type, pH 10, 30°C Petroselinum crispum
0.4
-
 (2E)-4-formyl-cinnamic acid wild-type, pH 10, 30°C Petroselinum crispum
0.5
-
 L-phenylalanine mutant F137V, pH 10, 30°C Petroselinum crispum
0.5
-
 (2E)-4-methyl-cinnamic acid wild-type, pH 10, 30°C Petroselinum crispum
0.6
-
 L-phenylalanine wild-type, pH 10, 30°C Petroselinum crispum
0.7
-
 (2E)-4-fluoro-cinnamic acid mutant F137V, pH 10, 30°C Petroselinum crispum
2
-
 4-nitro-L-phenylalanine wild-type, pH 10, 30°C Petroselinum crispum
2.2
-
 4-nitro-L-phenylalanine mutant F137V, pH 10, 30°C Petroselinum crispum
2.5
-
 4-trifluoromethyl-L-phenylalanine mutant F137V, pH 10, 30°C Petroselinum crispum
2.8
-
 (2E)-4-nitro-cinnamic acid wild-type, pH 10, 30°C Petroselinum crispum
3.1
-
 4-trifluoromethyl-L-phenylalanine wild-type, pH 10, 30°C Petroselinum crispum
4.2
-
 (2E)-4-nitro-cinnamic acid mutant F137V, pH 10, 30°C Petroselinum crispum
5.9
-
 (2E)-4-hydroxycinnamate mutant F137V, pH 10, 30°C Petroselinum crispum
12.6
-
 (2E)-4-hydroxycinnamate wild-type, pH 10, 30°C Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-4-amino-cinnamic acid + NH3 poor substrate Petroselinum crispum 4-amino-L-phenylalanine
-
 r
(2E)-4-fluoro-cinnamic acid + NH3
-
 Petroselinum crispum 4-fluoro-L-phenylalanine
-
 r
(2E)-4-formyl-cinnamic acid + NH3
-
 Petroselinum crispum 4-formyl-L-phenylalanine
-
 r
(2E)-4-hydroxycinnamate + NH3
-
 Petroselinum crispum L-tyrosine
-
 r
(2E)-4-methyl-cinnamic acid + NH3
-
 Petroselinum crispum 4-methyl-L-phenylalanine
-
 r
(2E)-4-nitro-cinnamic acid + NH3
-
 Petroselinum crispum 4-nitro-L-phenylalanine
-
 r
(2E)-4-trifluoromethyl-cinnamic acid + NH3 poor substrate Petroselinum crispum 4-trifluoromethyl-L-phenylalanine
-
 r
(E)-cinnamate + NH3
-
 Petroselinum crispum L-phenylalanine
-
 r
4-fluoro-L-phenylalanine
-
 Petroselinum crispum 4-fluoro-(E)-cinnamate + NH3
-
 r
4-nitro-L-phenylalanine
-
 Petroselinum crispum 4-nitro-(E)-cinnamate + NH3
-
 r
4-trifluoromethyl-L-phenylalanine
-
 Petroselinum crispum 4-trifluoromethyl-(E)-cinnamate + NH3
-
 r
L-phenylalanine
-
 Petroselinum crispum (E)-cinnamate + NH3
-
 r
L-tyrosine poor substrate Petroselinum crispum (2E)-4-hydroxycinnamate + NH3
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Petroselinum crispum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 assay at Petroselinum crispum