Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, analysis, and comparison of PAL isozymes, expression and phylogenetic analysis | Ephedra sinica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL3 | Ephedra sinica | |
0.144 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL2 | Ephedra sinica | |
0.152 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL1 | Ephedra sinica | |
0.18 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL4 | Ephedra sinica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, about, PAL isozymes, sequence calculation | Ephedra sinica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | Ephedra sinica | in ephedrine alkaloid biosynthesis in Ephedra, trans-cinnamic acid produced from a PAL reaction is converted into benzoic acid or benzoyl-CoA by beta-oxidation, branching off from the common phenylpropanoid pathway, biosynthetic pathway of ephedrine alkaloids, overview | (E)-cinnamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ephedra sinica | B5U983 | PAL1; several pal genes encode at least four isoforms of PAL | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the expression of pal genes in the roots is higher than in the plants aerial parts, the activity of PAL in the roots is also higher than in the aerial parts | Ephedra sinica | - |
root | - |
Ephedra sinica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000115 | - |
aerial plant parts | Ephedra sinica |
0.0000783 | - |
roots | Ephedra sinica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Ephedra sinica | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | in ephedrine alkaloid biosynthesis in Ephedra, trans-cinnamic acid produced from a PAL reaction is converted into benzoic acid or benzoyl-CoA by beta-oxidation, branching off from the common phenylpropanoid pathway, biosynthetic pathway of ephedrine alkaloids, overview | Ephedra sinica | (E)-cinnamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, about, PAL isozymes, sequence calculation | Ephedra sinica |
Synonyms | Comment | Organism |
---|---|---|
More | Ephedra sinica and and Pinus sp. PAL form a gymnosperm-type PAL subfamily | Ephedra sinica |
PAL | - |
Ephedra sinica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ephedra sinica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.362 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL3 | Ephedra sinica | |
0.457 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL2 | Ephedra sinica | |
0.516 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL4 | Ephedra sinica | |
0.588 | - |
L-phenylalanine | pH 8.5, 37°C, isozyme PAL1 | Ephedra sinica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Ephedra sinica |