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Literature summary for 4.3.1.24 extracted from
- Biochemical characterization of a prokaryotic phenylalanine ammonia lyase (2005), J. Bacteriol., 187, 4286-4289.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into expression plasmid pHIS8. The recombinant EncP is overexpressed as an N-terminal octahistidyl-tagged fusion protein in Escherichia coli BL21 | Streptomyces maritimus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| V83A | mutant is more active than wild-type enzyme, turnover-number for L-Phe is 20.8fold higher than wild-type value, Km-value for L-Phe is 5.2fold higher than wild-type value | Streptomyces maritimus |
| V83H | inactive mutant | Streptomyces maritimus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-aminoindan-2-phosphonic acid | - |
Streptomyces maritimus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
L-Phe | pH 8.0, 30°C, wild-type enzyme | Streptomyces maritimus | |
| 0.12 | - |
L-Phe | pH 8.0, 30°C, mutant enzyme V83A | Streptomyces maritimus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Streptomyces maritimus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces maritimus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Streptomyces maritimus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe | - |
Streptomyces maritimus | (E)-cinnamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Streptomyces maritimus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EncP | - |
Streptomyces maritimus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.79 | - |
L-Phe | pH 8.0, 30°C, wild-type enzyme | Streptomyces maritimus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces maritimus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: about 70% of maximal activity, pH 9.0: about 70% of maximal activity | Streptomyces maritimus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00191 | - |
2-aminoindan-2-phosphonic acid | - |
Streptomyces maritimus | |
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Release 2023.1 (January 2023)
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