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Literature summary for 4.3.1.24 extracted from

  • Appert, C.; Logermann, E.; Hahlbrock, K.; Schmid, J.; Amrhein, N.
    Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselinum crispum Nym.) (1994), Eur. J. Biochem., 225, 491.
    View publication on PubMed
Search for more literature for 4.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
fusion proteins with gluthatione S-transferase expressed in Escherichia coli Petroselinum crispum

Inhibitors

Inhibitors Comment Organism Structure
Cinnamic acid (E)-cinnamate, competitive Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 L-Phe isoenzyme PAL-4 Petroselinum crispum
0.0169
-
 L-Phe isoenzyme PAL-2 Petroselinum crispum
0.0172
-
 L-Phe isoenzyme PAL-1 Petroselinum crispum
0.0245
-
 L-Phe isoenzyme PAL-3 Petroselinum crispum
2.3
-
 L-Tyr isoenzyme PAL-2 Petroselinum crispum
2.5
-
 L-Tyr isoenzyme PAL-1 Petroselinum crispum
2.6
-
 L-Tyr isoenzyme PAL-4 Petroselinum crispum
7.8
-
 L-Tyr isoenzyme PAL-3 Petroselinum crispum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77828
-
 4 * 77828, calöculation from nucleotide sequence Petroselinum crispum
300000
-
 isoenzyme PAL 1, gel filtration Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
 Nym., 4 isoenzymes: PAL 1, PAL 2, PAL 3, PAL 4
-

Purification (Commentary)

Purification (Comment) Organism
-
 Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe
-
 Petroselinum crispum (E)-cinnamate + NH3
-
 ?
L-tyrosine L-Tyr Petroselinum crispum p-coumarate + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 77828, calöculation from nucleotide sequence Petroselinum crispum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
 Petroselinum crispum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 L-Tyr 30°C Petroselinum crispum
22
-
 L-Phe 30°C Petroselinum crispum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Petroselinum crispum