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Literature summary for 4.3.1.23 extracted from

  • Zhou, S.; Liu, P.; Chen, J.; Du, G.; Li, H.; Zhou, J.
    Characterization of mutants of a tyrosine ammonia-lyase from Rhodotorula glutinis (2016), Appl. Microbiol. Biotechnol., 100, 10443-10452 .
    View publication on PubMed
Search for more literature for 4.3.1.23

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and mutant enzymes are expressed with 6His fusion tags in Escherichia coli Rhodotorula glutinis

Protein Variants

Protein Variants Comment Organism
A11T/S106C/N170D/M227T/D542G trans-p-hydroxycinnamate production of the mutant enzyme is 41.2% higher than that observed from wild-type strain Rhodotorula glutinis
A11T/S106C/N170D/M227T/E518V trans-p-hydroxycinnamate production of the mutant enzyme is 38% higher than that observed from wild-type strain Rhodotorula glutinis
S9N/A125V trans-p-hydroxycinnamate production of the mutant enzyme is 37.1% higher than that observed from wild-type strain Rhodotorula glutinis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 L-tyrosine mutant enzyme A11T/S106C/N170D/M227T/E518V, pH 8.5, 40°C Rhodotorula glutinis
0.38
-
 L-tyrosine mutant enzyme S9N/A125V, pH 8.5, 40°C Rhodotorula glutinis
0.38
-
 L-tyrosine wild-type enzyme, pH 8.5, 40°C Rhodotorula glutinis
0.39
-
 L-tyrosine mutant enzyme A11T/S106C/N170D/M227T/D542G, pH 8.5, 40°C Rhodotorula glutinis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Rhodotorula glutinis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine Rhodotorula glutinis
-
 trans-p-hydroxycinnamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodotorula glutinis U5TV35 cf. EC 4.2.1.24
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine
-
 Rhodotorula glutinis trans-p-hydroxycinnamate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Rhodotorula glutinis

Synonyms

Synonyms Comment Organism
TAL
-
 Rhodotorula glutinis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Rhodotorula glutinis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
107
-
 L-tyrosine mutant enzyme A11T/S106C/N170D/M227T/E518V, pH 8.5, 40°C Rhodotorula glutinis
114
-
 L-tyrosine wild-type enzyme, pH 8.5, 40°C Rhodotorula glutinis
115
-
 L-tyrosine mutant enzyme S9N/A125V, pH 8.5, 40°C Rhodotorula glutinis
139
-
 L-tyrosine mutant enzyme A11T/S106C/N170D/M227T/D542G, pH 8.5, 40°C Rhodotorula glutinis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Rhodotorula glutinis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
298
-
 L-tyrosine wild-type enzyme, pH 8.5, 40°C Rhodotorula glutinis
303
-
 L-tyrosine mutant enzyme S9N/A125V, pH 8.5, 40°C Rhodotorula glutinis
355
-
 L-tyrosine mutant enzyme A11T/S106C/N170D/M227T/E518V, pH 8.5, 40°C Rhodotorula glutinis
361
-
 L-tyrosine mutant enzyme A11T/S106C/N170D/M227T/D542G, pH 8.5, 40°C Rhodotorula glutinis