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Literature summary for 4.3.1.2 extracted from

  • Asuncion, M.; Barlow, J.N.; Pollard, J.; Staines, A.G.; McMahon, S.A.; Blankenfeldt, W.; Gani, D.; Naismith, J.H.
    Overexpression, purification, crystallization and data collection of 3-methylaspartase from Clostridium tetanomorphum (2001), Acta Crystallogr. Sect. D, 57, 731-733.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium tetanomorphum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion, 0.0045 ml protein sample and 0.0045 ml precipitant, crystals are obtained using 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl as precipitant solution and 16-22% ethylene glycol as additive, crystals diffract to 2.0 A Clostridium tetanomorphum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
L-threo-3-methylaspartate recombinant 3-methylaspartase Clostridium tetanomorphum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45538
-
2 * 45538, mass spectrometry Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-erythro-3-methylaspartate Clostridium tetanomorphum i.e. (2S,3R)-3-methylaspartate, slow syn elimination mesaconate + NH3
-
r
L-threo-3-methylaspartate Clostridium tetanomorphum
-
mesaconate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 3-methylaspartase Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-erythro-3-methylaspartate i.e. (2S,3R)-3-methylaspartate, slow syn elimination Clostridium tetanomorphum mesaconate + NH3
-
?
L-erythro-3-methylaspartate i.e. (2S,3R)-3-methylaspartate, slow syn elimination Clostridium tetanomorphum mesaconate + NH3
-
r
L-threo-3-methylaspartate
-
Clostridium tetanomorphum mesaconate + NH3
-
r
L-threo-3-methylaspartate i.e. (2S,3S)-3-methylaspartate Clostridium tetanomorphum mesaconate + NH3
-
r

Subunits

Subunits Comment Organism
dimer 2 * 45538, mass spectrometry Clostridium tetanomorphum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
240
-
L-threo-3-methylaspartate recombinant 3-methylaspartase Clostridium tetanomorphum