Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridium tetanomorphum |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion, 0.0045 ml protein sample and 0.0045 ml precipitant, crystals are obtained using 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl as precipitant solution and 16-22% ethylene glycol as additive, crystals diffract to 2.0 A | Clostridium tetanomorphum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
L-threo-3-methylaspartate | recombinant 3-methylaspartase | Clostridium tetanomorphum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45538 | - |
2 * 45538, mass spectrometry | Clostridium tetanomorphum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-erythro-3-methylaspartate | Clostridium tetanomorphum | i.e. (2S,3R)-3-methylaspartate, slow syn elimination | mesaconate + NH3 | - |
r | |
L-threo-3-methylaspartate | Clostridium tetanomorphum | - |
mesaconate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant 3-methylaspartase | Clostridium tetanomorphum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-erythro-3-methylaspartate | i.e. (2S,3R)-3-methylaspartate, slow syn elimination | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
L-erythro-3-methylaspartate | i.e. (2S,3R)-3-methylaspartate, slow syn elimination | Clostridium tetanomorphum | mesaconate + NH3 | - |
r | |
L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | mesaconate + NH3 | - |
r | |
L-threo-3-methylaspartate | i.e. (2S,3S)-3-methylaspartate | Clostridium tetanomorphum | mesaconate + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45538, mass spectrometry | Clostridium tetanomorphum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
L-threo-3-methylaspartate | recombinant 3-methylaspartase | Clostridium tetanomorphum |