Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ile | feed-back inhibition | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
L-Thr | - |
Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | 115000 | gel filtration | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
X-1 | - |
Thermus sp. X-1 | - |
X-1 | - |
Purification (Comment) | Organism |
---|---|
partial | Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermus sp. |
Storage Stability | Organism |
---|---|
-20°C, 0.05 M potassium phosphate, pH 7.2, enzyme concentration 0.06 mg/ml, half-life: 4 weeks | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Thermus sp. | pyruvate + NH3 | - |
? | |
L-serine | - |
Thermus sp. X-1 | pyruvate + NH3 | - |
? | |
L-threonine | - |
Thermus sp. | 2-oxobutanoate + NH3 | - |
? | |
L-threonine | - |
Thermus sp. X-1 | 2-oxobutanoate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 90 | - |
Thermus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 98 | 60°C: about 30% of maximal activity, 98°C: about 15% of maximal activity | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, 15% loss of activity | Thermus sp. |
90 | - |
rapid inactivation | Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Thermus sp. |