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Literature summary for 4.3.1.19 extracted from

  • Kumagai, H.; Nishimura, T.; Yamada, H.
    Biodegradative threonine deaminase from Proteus morganii (1978), Agric. Biol. Chem., 42, 613-621.
No PubMed abstract available
Search for more literature for 4.3.1.19

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Morganella morganii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 L-Thr in presence of 1 mM AMP Morganella morganii
8.9
-
 L-Ser in presence of AMP Morganella morganii
24
-
 L-Thr in absence of AMP Morganella morganii
30.3
-
 L-Ser in absence of AMP Morganella morganii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 4 * 32000, SDS-PAGE Morganella morganii
120000
-
 gel filtration Morganella morganii

Organism

Organism UniProt Comment Textmining
Morganella morganii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Morganella morganii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.6
-
-
 Morganella morganii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-Ala
-
 Morganella morganii ?
-
 ?
L-serine
-
 Morganella morganii pyruvate + NH3
-
 ?
L-threonine
-
 Morganella morganii 2-oxobutanoate + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Morganella morganii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5 in presence of 1 mM AMP Morganella morganii
9
-
 in absence of AMP Morganella morganii

Cofactor

Cofactor Comment Organism Structure
ADP activates Morganella morganii
AMP activates Morganella morganii
pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Morganella morganii