Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
Y203A | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 14% (wild-type 100%) | Saccharomyces cerevisiae |
Y203D | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 0.57% (wild-type 100%) | Saccharomyces cerevisiae |
Y203E | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 0.47% (wild-type 100%) | Saccharomyces cerevisiae |
Y203F | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 25% (wild-type 100%) | Saccharomyces cerevisiae |
Y203R | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 2.9% (wild-type 100%) | Saccharomyces cerevisiae |
Y203S | Km increased compared to wild-type, kcat decreased compared to wild-type. kcat ratio: 14% (wild-type 100%) | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-serine | pH 8, 30°C, wild-type | Saccharomyces cerevisiae | |
0.7 | - |
D-serine | pH 8, 30°C, mutant Y203F | Saccharomyces cerevisiae | |
1.8 | - |
D-serine | pH 8, 30°C, mutant Y203A | Saccharomyces cerevisiae | |
3.3 | - |
D-serine | pH 8, 30°C, mutant Y203S | Saccharomyces cerevisiae | |
5.1 | - |
D-serine | pH 8, 30°C, mutant Y203R | Saccharomyces cerevisiae | |
5.6 | - |
D-serine | pH 8, 30°C, mutant Y203E | Saccharomyces cerevisiae | |
8.5 | - |
D-serine | pH 8, 30°C, mutant Y203D | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53095 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine | - |
Saccharomyces cerevisiae | pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-serine dehydratase | - |
Saccharomyces cerevisiae |
Dsd1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
D-serine | pH 8, 30°C, mutant Y203E | Saccharomyces cerevisiae | |
0.097 | - |
D-serine | pH 8, 30°C, mutant Y203D | Saccharomyces cerevisiae | |
0.5 | - |
D-serine | pH 8, 30°C, mutant Y203R | Saccharomyces cerevisiae | |
2.3 | - |
D-serine | pH 8, 30°C, mutant Y203A | Saccharomyces cerevisiae | |
2.3 | - |
D-serine | pH 8, 30°C, mutant Y203S | Saccharomyces cerevisiae | |
4.3 | - |
D-serine | pH 8, 30°C, mutant Y203F | Saccharomyces cerevisiae | |
17 | - |
D-serine | pH 8, 30°C, wild-type | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
D-serine | pH 8, 30°C, mutant Y203D | Saccharomyces cerevisiae | |
0.015 | - |
D-serine | pH 8, 30°C, mutant Y203E | Saccharomyces cerevisiae | |
0.1 | - |
D-serine | pH 8, 30°C, mutant Y203R | Saccharomyces cerevisiae | |
0.68 | - |
D-serine | pH 8, 30°C, mutant Y203S | Saccharomyces cerevisiae | |
1.2 | - |
D-serine | pH 8, 30°C, mutant Y203A | Saccharomyces cerevisiae | |
6.1 | - |
D-serine | pH 8, 30°C, mutant Y203F | Saccharomyces cerevisiae | |
90 | - |
D-serine | pH 8, 30°C, wild-type | Saccharomyces cerevisiae |