Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
L-serine | pH 7, temperature not specified in the publication | Legionella pneumophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49476 | - |
gel filtration, 1 * 49476, monomeric form does not possess enzymatic activity | Legionella pneumophila |
49476 | - |
gel filtration, 2 * 49476, only dimer displays catalytic activity. In the absence of substrate, lpLSD is predominately a dimer | Legionella pneumophila |
49480 | - |
gel filtration, monomer | Legionella pneumophila |
98950 | - |
gel filtration, dimer | Legionella pneumophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using cobalt-based Talon immobilized metal affinity columns | Legionella pneumophila |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine = pyruvate + NH3 | pre-steady-state kinetic analysis of L-serine binding to lpLSD demonstrates that L-serine binds to a second noncatalytic site and produces a conformational change in the enzyme. The rate of this conformational change is too slow for its participation in the catalytic cycle but rather occurs prior to catalysis to produce an activated form of the enzyme | Legionella pneumophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Legionella pneumophila | pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration, 2 * 49476, only dimer displays catalytic activity. In the absence of substrate, lpLSD is predominately a dimer | Legionella pneumophila |
monomer | gel filtration, 1 * 49476, monomeric form does not possess enzymatic activity | Legionella pneumophila |
Synonyms | Comment | Organism |
---|---|---|
lpLSD | - |
Legionella pneumophila |