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Literature summary for 4.3.1.17 extracted from
- Characterization of two isotypes of L-threonine dehydratase from Entamoeba histolytica (2010), Mol. Biochem. Parasitol., 170, 100-104.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged version expressed in Escherichia coli BL21(DE3) | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxobutyrate | 20 mM: 49.3% inhibition, not reversed by 5 mM AMP | Entamoeba histolytica |  |
| ATP | - |
Entamoeba histolytica | |
| glyoxylate | 20 mM: 54.1% inhibition, not reversed by 5 mM AMP | Entamoeba histolytica | |
| L-cysteine | competitive | Entamoeba histolytica | |
| pyruvate | 20 mM: 45.3% inhibition, not reversed by 5 mM AMP | Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.3 | - |
L-threonine | - |
Entamoeba histolytica | |
| 13.5 | - |
L-serine | - |
Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, calculated | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | B1N2N4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine | - |
Entamoeba histolytica | pyruvate + NH3 | - |
? | |
| L-threonine | - |
Entamoeba histolytica | 2-oxobutyrate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, calculated | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-serine dehydratase | - |
Entamoeba histolytica |
| L-threonine dehydratase | - |
Entamoeba histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
L-serine | - |
Entamoeba histolytica | |
| 5.2 | - |
L-threonine | - |
Entamoeba histolytica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Entamoeba histolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Entamoeba histolytica | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
L-cysteine | substrate L-serine | Entamoeba histolytica | |
| 2.2 | - |
L-cysteine | substrate L-threonine | Entamoeba histolytica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Entamoeba histolytica | calculated | - |
6.68 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 10 | - |
- |
Entamoeba histolytica | ATP | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
L-serine | - |
Entamoeba histolytica | |
| 0.7 | - |
L-threonine | - |
Entamoeba histolytica | |
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Release 2023.1 (January 2023)
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