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Literature summary for 4.3.1.17 extracted from

  • Cicchillo, R.M.; Baker, M.A.; Schnitzer, E.J.; Newman, E.B.; Krebs, C.; Booker, S.J.
    Escherichia coli L-serine deaminase requires a [4Fe-4S] cluster in catalysis (2004), J. Biol. Chem., 279, 32418-32425.
    View publication on PubMed
Search for more literature for 4.3.1.17

General Stability

General Stability Organism
it is absolutely critical that dilutions of the purified enzyme be made in buffer containing 50% glycerol, otherwise the enzyme rapidly loses activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
D-serine competitive Escherichia coli
L-alanine
-
 Escherichia coli
L-cysteine competitive Escherichia coli
additional information not inhibitory: L-alanine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.67
-
 L-serine pH 8.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron presence of (4Fe-4S)2+ and of (2Fe-2S)2+ clusters involved in catalytic turnover Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 51000, His-tagged protein, SDS-PAGE and calculated Escherichia coli
107000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purification under anaerobic conditions Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-threonine at 4.6% of the rate with L-serine Escherichia coli 2-oxobutyrate + NH3
-
 ?
L-serine
-
 Escherichia coli pyruvate + NH3
-
 ?
L-threonine at 2.7% of the rate with L-serine Escherichia coli 2-oxobutyrate + NH3
-
 ?
additional information no substrate: D-serine. Extremely poor substrate: L-cysteine Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 51000, His-tagged protein, SDS-PAGE and calculated Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
436
-
 L-serine pH 8.0, 37°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.9
-
 L-alanine pH 8.0, 37°C Escherichia coli
1.41
-
 D-serine pH 8.0, 37°C Escherichia coli