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Literature summary for 4.3.1.17 extracted from

  • Kubota, K.; Yokozeki, K.; Ozaki, H.
    Effect of L-serine dehydratase activity onL-serine production by Corynebacterium glycinophilum and an examination of the properties of the enzyme (1989), J. Ferment. Bioeng., 67, 391-394.
No PubMed abstract available
Search for more literature for 4.3.1.17

Inhibitors

Inhibitors Comment Organism Structure
Gly
-
 Corynebacterium glyciniphilum
L-Cys
-
 Corynebacterium glyciniphilum
L-Trp
-
 Corynebacterium glyciniphilum
L-Val
-
 Corynebacterium glyciniphilum
MnCl2
-
 Corynebacterium glyciniphilum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ slight activation by FeCl2 Corynebacterium glyciniphilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 gel filtration Corynebacterium glyciniphilum

Organism

Organism UniProt Comment Textmining
Corynebacterium glyciniphilum
-
 various mutants
-
Corynebacterium glyciniphilum AJ-3170
-
 various mutants
-

Purification (Commentary)

Purification (Comment) Organism
strain AJ-3170 Corynebacterium glyciniphilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.262
-
-
 Corynebacterium glyciniphilum

Storage Stability

Storage Stability Organism
-20°C, 40% loss of activity after 24 h, 0.76 mg/ml protein concentration, partially purified enzyme Corynebacterium glyciniphilum
4°C, 90% loss of activity after 24 h, 0.76 mg/ml protein concentration, partially purified enzyme Corynebacterium glyciniphilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu 3% of the activity with L-Ser Corynebacterium glyciniphilum 3-Hydroxy-2-oxopropionate + NH3
-
 ?
L-Leu 3% of the activity with L-Ser Corynebacterium glyciniphilum AJ-3170 3-Hydroxy-2-oxopropionate + NH3
-
 ?
L-serine specific for L-Ser Corynebacterium glyciniphilum pyruvate + NH3
-
 ?
L-serine specific for L-Ser Corynebacterium glyciniphilum AJ-3170 pyruvate + NH3
-
 ?
L-Thr 1% of the activity with L-Ser Corynebacterium glyciniphilum 2-oxobutanoate + NH3
-
 ?
L-Thr 1% of the activity with L-Ser Corynebacterium glyciniphilum AJ-3170 2-oxobutanoate + NH3
-
 ?
L-Trp 17% of the activity with L-Ser Corynebacterium glyciniphilum ?
-
 ?
L-Trp 17% of the activity with L-Ser Corynebacterium glyciniphilum AJ-3170 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Corynebacterium glyciniphilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 50 18°C: about 50% of maximal activity, 50°C: about 25% of maximal activity Corynebacterium glyciniphilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Corynebacterium glyciniphilum

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7: about 35% of maximal activity, pH 9.5: about 35% of maximal activity Corynebacterium glyciniphilum