Application | Comment | Organism |
---|---|---|
synthesis | the recombinant enzyme expressed in Escherichia coli is used to produce optically pure L-erythro-3-hydroxyaspartate and D-threo-3-hydroxyaspartate from the corresponding DL-racemic mixtures | Pseudomonas sp. N99 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. N99 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
erythro-3-hydroxy-D-aspartate | pH and temperature not specified in the publication | Pseudomonas sp. N99 | |
0.57 | - |
threo-3-hydroxy-L-aspartate | pH and temperature not specified in the publication | Pseudomonas sp. N99 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39100 | - |
SDS-PAGE | Pseudomonas sp. N99 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. N99 | L0N5C4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme | Pseudomonas sp. N99 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.54 | - |
pH and temperature not specified in the publication | Pseudomonas sp. N99 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
erythro-3-hydroxy-D-aspartate | - |
Pseudomonas sp. N99 | oxaloacetate + NH3 | - |
? | |
additional information | no activity with erythro-3-hydroxy-L-aspartate and threo-3-hydroxy-D-aspartate | Pseudomonas sp. N99 | ? | - |
? | |
threo-3-hydroxy-L-aspartate | - |
Pseudomonas sp. N99 | oxaloacetate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39100, SDS-PAGE | Pseudomonas sp. N99 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Pseudomonas sp. N99 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
15 min, the enzyme is stable below 25°C | Pseudomonas sp. N99 |
45 | - |
15 min, the enzyme lost its activity above 45°C | Pseudomonas sp. N99 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.19 | - |
erythro-3-hydroxy-D-aspartate | pH and temperature not specified in the publication | Pseudomonas sp. N99 | |
8.61 | - |
threo-3-hydroxy-L-aspartate | pH and temperature not specified in the publication | Pseudomonas sp. N99 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant enzyme | Pseudomonas sp. N99 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.01 | - |
erythro-3-hydroxy-D-aspartate | pH and temperature not specified in the publication | Pseudomonas sp. N99 | |
15.2 | - |
threo-3-hydroxy-L-aspartate | pH and temperature not specified in the publication | Pseudomonas sp. N99 |