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Literature summary for 4.3.1.16 extracted from

  • Nagano, H.; Shibano, K.; Matsumoto, Y.; Yokota, A.; Wada, M.
    Isolation and amino acid sequence of a dehydratase acting on D-erythro-3-hydroxyaspartate from Pseudomonas sp. N99, and its application in the production of optically active 3-hydroxyaspartate (2017), Biosci. Biotechnol. Biochem., 81, 1156-1164 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the recombinant enzyme expressed in Escherichia coli is used to produce optically pure L-erythro-3-hydroxyaspartate and D-threo-3-hydroxyaspartate from the corresponding DL-racemic mixtures Pseudomonas sp. N99

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp. N99

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
erythro-3-hydroxy-D-aspartate pH and temperature not specified in the publication Pseudomonas sp. N99
0.57
-
threo-3-hydroxy-L-aspartate pH and temperature not specified in the publication Pseudomonas sp. N99

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39100
-
SDS-PAGE Pseudomonas sp. N99

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. N99 L0N5C4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Pseudomonas sp. N99

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.54
-
pH and temperature not specified in the publication Pseudomonas sp. N99

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
erythro-3-hydroxy-D-aspartate
-
Pseudomonas sp. N99 oxaloacetate + NH3
-
?
additional information no activity with erythro-3-hydroxy-L-aspartate and threo-3-hydroxy-D-aspartate Pseudomonas sp. N99 ?
-
?
threo-3-hydroxy-L-aspartate
-
Pseudomonas sp. N99 oxaloacetate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 39100, SDS-PAGE Pseudomonas sp. N99

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant enzyme Pseudomonas sp. N99

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
15 min, the enzyme is stable below 25°C Pseudomonas sp. N99
45
-
15 min, the enzyme lost its activity above 45°C Pseudomonas sp. N99

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.19
-
erythro-3-hydroxy-D-aspartate pH and temperature not specified in the publication Pseudomonas sp. N99
8.61
-
threo-3-hydroxy-L-aspartate pH and temperature not specified in the publication Pseudomonas sp. N99

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
recombinant enzyme Pseudomonas sp. N99

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.01
-
erythro-3-hydroxy-D-aspartate pH and temperature not specified in the publication Pseudomonas sp. N99
15.2
-
threo-3-hydroxy-L-aspartate pH and temperature not specified in the publication Pseudomonas sp. N99