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Literature summary for 4.3.1.15 extracted from
- Studies on a 2,3-diaminopropionate:ammonia-lyase from a pseudomonad (1975), Hoppe-Seyler's Z. Physiol. Chem., 356, 193-201.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Iodosylbenzoate | - |
Pseudomonas sp. |  |
| hydroxylamine | - |
Pseudomonas sp. | |
| NaCN | - |
Pseudomonas sp. | |
| NaHSO3 | - |
Pseudomonas sp. | |
| NEM | - |
Pseudomonas sp. | |
| PCMB | - |
Pseudomonas sp. | |
| Semicarbazide | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Pseudomonas sp. |
| 80000 | - |
gel filtration | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-2,3-Diaminopropanoate + H2O | only 12% of the activity with L-2,3-diaminopropanoate | Pseudomonas sp. | Pyruvate + NH3 | - |
? | |
| L-2,3-Diaminopropanoate + H2O | - |
Pseudomonas sp. | Pyruvate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
partial inactivation | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | contains 1 mol of pyridoxal 5'-phosphate per mol of enzyme | Pseudomonas sp. | |
| pyridoxal 5'-phosphate | Km: 0.025 mM | Pseudomonas sp. | |
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Release 2023.1 (January 2023)
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