BRENDA - Enzyme Database
show all sequences of 4.3.1.14

Identification of the last unknown genes in the fermentation pathway of lysine

Kreimeyer, A.; Perret, A.; Lechaplais, C.; Vallenet, D.; Medigue, C.; Salanoubat, M.; Weissenbach, J.; J. Biol. Chem. 282, 7191-7197 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
lysine fermentation pathway
Anaerobic bacteria
Cloned(Commentary)
Commentary
Organism
in Escherichia coli
Anaerobic bacteria
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
55
-
crotonyl-CoA
-
Anaerobic bacteria
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
SDS-PAGE
Anaerobic bacteria
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Anaerobic bacteria
-
environmental sample obtained from anaerobic digesters, putative lysine-fermenting bacteria like Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. vincentii, Thermoanaerobacter tengcongensis, Porphyromonas gingivalis, Alkaliphilus metalliredigenes, Myxococcus xanthus, Anaeromyxobacter dehlogenans, Clostridium sp., Rhodoferax ferrireducens, Symbiobacterium thermophilum, Nocarioides, Janibacter sp.
-
Purification (Commentary)
Commentary
Organism
of the recombinant proteins by HiPrep 26/10 desalting column chromatography
Anaerobic bacteria
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-3-Aminobutanoyl-CoA
-
680885
Anaerobic bacteria
Crotonyl-CoA + NH3
-
-
-
r
Application (protein specific)
Application
Commentary
Organism
biotechnology
lysine fermentation pathway
Anaerobic bacteria
Cloned(Commentary) (protein specific)
Commentary
Organism
in Escherichia coli
Anaerobic bacteria
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
55
-
crotonyl-CoA
-
Anaerobic bacteria
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
SDS-PAGE
Anaerobic bacteria
Purification (Commentary) (protein specific)
Commentary
Organism
of the recombinant proteins by HiPrep 26/10 desalting column chromatography
Anaerobic bacteria
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-3-Aminobutanoyl-CoA
-
680885
Anaerobic bacteria
Crotonyl-CoA + NH3
-
-
-
r
Other publictions for EC 4.3.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
680885
Kreimeyer
Identification of the last unk ...
Anaerobic bacteria
J. Biol. Chem.
282
7191-7197
2007
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1
1
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1
-
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1
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4
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1
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1
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-
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-
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1
1
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1
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1
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1
-
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
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3378
Barker
Pathway of lysine degradation ...
Fusobacterium nucleatum
J. Bacteriol.
152
201-207
1982
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-
-
-
-
-
-
-
-
-
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1
-
1
-
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1
-
-
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2
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1
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1
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2
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-
-
-
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6044
Barker
Enzymes involved in 3,5-diamin ...
Brevibacterium sp.
J. Bacteriol.
143
1165-1170
1980
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-
-
-
-
-
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2
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1
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1
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4
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2
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1
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4
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6045
Jeng
Purification and properties of ...
Clostridium sp., Clostridium sp. SB4
J. Biol. Chem.
249
6578-6584
1974
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-
-
-
-
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9
3
-
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1
2
-
14
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-
1
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1
1
10
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-
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1
-
2
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-
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-
-
9
-
3
-
-
1
2
-
-
-
1
-
-
1
1
10
-
-
-
1
-
2
-
-
-
-
-
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