General Stability | Organism |
---|---|
ornithine, NAD+ and dithiothreitol stabilize | Clostridium sporogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | - |
Clostridium sporogenes | |
N-ethylmaleimide | 2 mM, complete inhibition | Clostridium sporogenes | |
O2 | - |
Clostridium sporogenes | |
p-chloromercuribenzoate | 1 mM, complete inhibition | Clostridium sporogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
L-ornithine | in the absence of ADP | Clostridium sporogenes | |
6.2 | - |
L-ornithine | in the presence of ADP | Clostridium sporogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
2 * 41500, SDS-PAGE | Clostridium sporogenes |
80220 | - |
amino acid analysis | Clostridium sporogenes |
81000 | - |
equilibrium sedimentation | Clostridium sporogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | Clostridium sporogenes | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sporogenes | - |
National Canners Association, PA 3679 | - |
Clostridium sporogenes | - |
ATCC 7955 | - |
Oxidation Stability | Organism |
---|---|
addition of dithiothreitol and subsequent incubation in argon atmosphere completely reverses the inhibition caused by oxygen | Clostridium sporogenes |
enzyme is sensitive to either pure oxygen or air | Clostridium sporogenes |
Purification (Comment) | Organism |
---|---|
- |
Clostridium sporogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium sporogenes |
6.84 | - |
- |
Clostridium sporogenes |
Storage Stability | Organism |
---|---|
4°C, 50% loss of activity under argon atmosphere within 3 days | Clostridium sporogenes |
stable for at least 1 year when stored in sealed ampoules in argon atmosphere in liquid nitrogen | Clostridium sporogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | anaerobic | Clostridium sporogenes | L-proline + NH3 | - |
? | |
L-ornithine | - |
Clostridium sporogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41500, SDS-PAGE | Clostridium sporogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | stimulates | Clostridium sporogenes | |
ATP | stimulates | Clostridium sporogenes | |
NAD+ | tightly bound | Clostridium sporogenes | |
NAD+ | 1 mol per mol of enzyme | Clostridium sporogenes |