Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
D-serine O-sulfate | enzyme B | Pseudomonas sp. | |
0.17 | - |
D-Cystine | enzyme B | Pseudomonas sp. | |
1.1 | - |
S-Benzyl-L-cysteine | enzyme A | Pseudomonas sp. | |
1.3 | - |
O-benzyl-L-serine | enzyme A | Pseudomonas sp. | |
2 | - |
L-cystine | enzyme A | Pseudomonas sp. | |
2 | - |
O-benzyl-DL-serine | enzyme B, calculated on the basis of the non-reactivity of the L-isomer | Pseudomonas sp. | |
2.2 | - |
D-cystathionine | enzyme B | Pseudomonas sp. | |
5.4 | - |
L-serine O-sulfate | enzyme B | Pseudomonas sp. | |
7.9 | - |
L-serine O-sulfate | enzyme A | Pseudomonas sp. | |
8.3 | - |
D-serine | enzyme B | Pseudomonas sp. | |
8.5 | - |
L-cystathionine | enzyme B | Pseudomonas sp. | |
9.1 | - |
L-cystathionine | enzyme A | Pseudomonas sp. | |
17.8 | - |
S-methyl-L-cysteine | enzyme A | Pseudomonas sp. | |
20 | - |
O-acetyl-L-serine | enzyme A | Pseudomonas sp. | |
38 | - |
S-ethyl-L-cysteine | enzyme A | Pseudomonas sp. | |
56 | - |
S-methyl-L-cysteine | enzyme B | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas sp. | Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) | ? | - |
? | |
additional information | Pseudomonas sp. FR | Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) | ? | - |
? | |
S-methyl-L-cysteine | Pseudomonas sp. | growth pattern is similar to that observed with L-serine O-sulfate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
FR | - |
Pseudomonas sp. FR | - |
FR | - |
Purification (Comment) | Organism |
---|---|
enzyme A | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-L-alanine methyl ester | 3-chloro-L-alanine | Pseudomonas sp. | ? | - |
? | |
3-chloro-L-alanine methyl ester | 3-chloro-L-alanine | Pseudomonas sp. FR | ? | - |
? | |
3-cyano-L-alanine | - |
Pseudomonas sp. | ? | - |
? | |
beta-ureido-alpha-aminopropionic acid | - |
Pseudomonas sp. | ? | - |
? | |
D-cystathionine | - |
Pseudomonas sp. | ? | - |
? | |
D-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
D-cystine | - |
Pseudomonas sp. | ? | - |
? | |
D-serine | - |
Pseudomonas sp. | ? | - |
? | |
D-serine-O-sulfate | - |
Pseudomonas sp. | pyruvate + NH3 + SO42- | - |
? | |
DL-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
DL-lanthionine | - |
Pseudomonas sp. | ? | - |
? | |
L-cystathionine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
L-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
L-cystine | - |
Pseudomonas sp. | ? | - |
? | |
L-serine | - |
Pseudomonas sp. | ? | - |
? | |
L-serine O-sulfate | - |
Pseudomonas sp. | pyruvate + NH3 + sulfate | - |
? | |
L-serine O-sulfate | - |
Pseudomonas sp. FR | pyruvate + NH3 + sulfate | - |
? | |
L-threonine O-sulfate | - |
Pseudomonas sp. | 2-oxobutanoate + NH3 + SO42- | - |
? | |
L-threonine O-sulfate | - |
Pseudomonas sp. FR | 2-oxobutanoate + NH3 + SO42- | - |
? | |
additional information | multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers | Pseudomonas sp. | ? | - |
? | |
additional information | Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) | Pseudomonas sp. | ? | - |
? | |
additional information | multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers | Pseudomonas sp. FR | ? | - |
? | |
additional information | Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) | Pseudomonas sp. FR | ? | - |
? | |
O-acetyl-L-serine | - |
Pseudomonas sp. | ? | - |
? | |
O-benzyl DL-serine | - |
Pseudomonas sp. | ? | - |
? | |
O-benzyl-L-serine | - |
Pseudomonas sp. | ? | - |
? | |
O-diazoacetyl-L-serine | - |
Pseudomonas sp. | ? | - |
? | |
O-methyl DL-serine | - |
Pseudomonas sp. | ? | - |
? | |
O-succinyl-L-serine | - |
Pseudomonas sp. | ? | - |
? | |
S-benzyl-L-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
S-carbamoyl-L-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
S-ethyl-L-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
S-methyl L-cysteine | - |
Pseudomonas sp. | ? | - |
? | |
S-methyl-L-cysteine | growth pattern is similar to that observed with L-serine O-sulfate | Pseudomonas sp. | ? | - |
? | |
S-sulfo-L-cysteine | - |
Pseudomonas sp. | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine | Pseudomonas sp. |