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Literature summary for 4.3.1.10 extracted from

  • Tudball, N.; O'Neill, J.G.
    Substrate specificity of the L-serine O-sulphate degrading activities of Pseudomonas FR (1976), Biochim. Biophys. Acta, 429, 616-623.
    View publication on PubMed
Search for more literature for 4.3.1.10

Inhibitors

Inhibitors Comment Organism Structure
additional information
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 D-serine O-sulfate enzyme B Pseudomonas sp.
0.17
-
 D-Cystine enzyme B Pseudomonas sp.
1.1
-
 S-Benzyl-L-cysteine enzyme A Pseudomonas sp.
1.3
-
 O-benzyl-L-serine enzyme A Pseudomonas sp.
2
-
 L-cystine enzyme A Pseudomonas sp.
2
-
 O-benzyl-DL-serine enzyme B, calculated on the basis of the non-reactivity of the L-isomer Pseudomonas sp.
2.2
-
 D-cystathionine enzyme B Pseudomonas sp.
5.4
-
 L-serine O-sulfate enzyme B Pseudomonas sp.
7.9
-
 L-serine O-sulfate enzyme A Pseudomonas sp.
8.3
-
 D-serine enzyme B Pseudomonas sp.
8.5
-
 L-cystathionine enzyme B Pseudomonas sp.
9.1
-
 L-cystathionine enzyme A Pseudomonas sp.
17.8
-
 S-methyl-L-cysteine enzyme A Pseudomonas sp.
20
-
 O-acetyl-L-serine enzyme A Pseudomonas sp.
38
-
 S-ethyl-L-cysteine enzyme A Pseudomonas sp.
56
-
 S-methyl-L-cysteine enzyme B Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) ?
-
 ?
additional information Pseudomonas sp. FR Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) ?
-
 ?
S-methyl-L-cysteine Pseudomonas sp. growth pattern is similar to that observed with L-serine O-sulfate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 FR
-
Pseudomonas sp. FR
-
 FR
-

Purification (Commentary)

Purification (Comment) Organism
enzyme A Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-L-alanine methyl ester 3-chloro-L-alanine Pseudomonas sp. ?
-
 ?
3-chloro-L-alanine methyl ester 3-chloro-L-alanine Pseudomonas sp. FR ?
-
 ?
3-cyano-L-alanine
-
 Pseudomonas sp. ?
-
 ?
beta-ureido-alpha-aminopropionic acid
-
 Pseudomonas sp. ?
-
 ?
D-cystathionine
-
 Pseudomonas sp. ?
-
 ?
D-cysteine
-
 Pseudomonas sp. ?
-
 ?
D-cystine
-
 Pseudomonas sp. ?
-
 ?
D-serine
-
 Pseudomonas sp. ?
-
 ?
D-serine-O-sulfate
-
 Pseudomonas sp. pyruvate + NH3 + SO42-
-
 ?
DL-cysteine
-
 Pseudomonas sp. ?
-
 ?
DL-lanthionine
-
 Pseudomonas sp. ?
-
 ?
L-cystathionine + H2O
-
 Pseudomonas sp. ?
-
 ?
L-cysteine
-
 Pseudomonas sp. ?
-
 ?
L-cystine
-
 Pseudomonas sp. ?
-
 ?
L-serine
-
 Pseudomonas sp. ?
-
 ?
L-serine O-sulfate
-
 Pseudomonas sp. pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate
-
 Pseudomonas sp. FR pyruvate + NH3 + sulfate
-
 ?
L-threonine O-sulfate
-
 Pseudomonas sp. 2-oxobutanoate + NH3 + SO42-
-
 ?
L-threonine O-sulfate
-
 Pseudomonas sp. FR 2-oxobutanoate + NH3 + SO42-
-
 ?
additional information multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers Pseudomonas sp. ?
-
 ?
additional information Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) Pseudomonas sp. ?
-
 ?
additional information multi-substrate activity of the enzymes A and B with the enzyme A having a preferential activity for L-isomers and enzyme B for D-isomers Pseudomonas sp. FR ?
-
 ?
additional information Pseudomonas FR posses two distinct L-serine O-sulfate degrading enzymes (A and B) Pseudomonas sp. FR ?
-
 ?
O-acetyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
O-benzyl DL-serine
-
 Pseudomonas sp. ?
-
 ?
O-benzyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
O-diazoacetyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
O-methyl DL-serine
-
 Pseudomonas sp. ?
-
 ?
O-succinyl-L-serine
-
 Pseudomonas sp. ?
-
 ?
S-benzyl-L-cysteine
-
 Pseudomonas sp. ?
-
 ?
S-carbamoyl-L-cysteine
-
 Pseudomonas sp. ?
-
 ?
S-ethyl-L-cysteine
-
 Pseudomonas sp. ?
-
 ?
S-methyl L-cysteine
-
 Pseudomonas sp. ?
-
 ?
S-methyl-L-cysteine growth pattern is similar to that observed with L-serine O-sulfate Pseudomonas sp. ?
-
 ?
S-sulfo-L-cysteine
-
 Pseudomonas sp. ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine Pseudomonas sp.