Application | Comment | Organism |
---|---|---|
synthesis | bioproduction of L-aspartic acid and cinnamic acid. From an initial concentration of 1000 mM of fumarate and 30 mM of L-phenylalanine, the enzyme converts 0.395 mM L-aspartic acid and 3.47 mM cinnamic acid, respectively | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.346 | - |
L-aspartate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
1.71 | - |
L-phenylalanine | pH 8.0, 35°C | Pseudomonas aeruginosa | |
2000 | - |
fumarate | pH 8.0, 35°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme does not require metal ion for its activity. It is activated in the presence of metal ions. Mg2+ is the most effective | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTD7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.42 | - |
substrate: fumarate, pH 8.0, 35°C | Pseudomonas aeruginosa |
31.21 | - |
substrate: L-aspartate, pH 8.0, 35°C | Pseudomonas aeruginosa |
38.35 | - |
substrate: L-phenylalanine, pH 8.0, 35°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + NH3 | - |
Pseudomonas aeruginosa | L-aspartate | - |
r | |
L-aspartate | - |
Pseudomonas aeruginosa | fumarate + NH3 | - |
r | |
L-phenylalanine | - |
Pseudomonas aeruginosa | cinnamic acid + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
L-aspartate ammonia lyase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
deamination reaction | Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | 15°C: about 80% of maximal activity, 30°C: about 40% of maximal activity, 35: maximal activity, 40°C: about 90% of maximal activity, 50°C: about 30% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the enzyme retains 56% activity after 7 days of incubation | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.908 | - |
L-aspartate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
8.002 | - |
L-phenylalanine | pH 8., 35°C | Pseudomonas aeruginosa | |
13.64 | - |
fumarate | pH 8.0, 35°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
deamination reaction | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 80% of maximal activity, pH 8.0: maximal activity, pH 9.0: about 25% of maximal activity, deamination reaction | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
fumarate | pH 8.0, 35°C | Pseudomonas aeruginosa | |
4.65 | - |
L-phenylalanine | pH 8.0, 35°C | Pseudomonas aeruginosa | |
14.18 | - |
L-aspartate | pH 8.0, 35°C | Pseudomonas aeruginosa |