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Literature summary for 4.3.1.1 extracted from

  • Patel, A.T.; Akhani, R.C.; Patel, M.J.; Dedania, S.R.; Patel, D.H.
    Bioproduction of L-aspartic acid and cinnamic Acid by L-aspartate ammonia lyase from Pseudomonas aeruginosa PAO1 (2017), Appl. Biochem. Biotechnol., 182, 792-803 .
    View publication on PubMed

Application

Application Comment Organism
synthesis bioproduction of L-aspartic acid and cinnamic acid. From an initial concentration of 1000 mM of fumarate and 30 mM of L-phenylalanine, the enzyme converts 0.395 mM L-aspartic acid and 3.47 mM cinnamic acid, respectively Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.346
-
L-aspartate pH 8.0, 35°C Pseudomonas aeruginosa
1.71
-
L-phenylalanine pH 8.0, 35°C Pseudomonas aeruginosa
2000
-
fumarate pH 8.0, 35°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme does not require metal ion for its activity. It is activated in the presence of metal ions. Mg2+ is the most effective Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
recombinant His-tagged enzyme, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HTD7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.42
-
substrate: fumarate, pH 8.0, 35°C Pseudomonas aeruginosa
31.21
-
substrate: L-aspartate, pH 8.0, 35°C Pseudomonas aeruginosa
38.35
-
substrate: L-phenylalanine, pH 8.0, 35°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + NH3
-
Pseudomonas aeruginosa L-aspartate
-
r
L-aspartate
-
Pseudomonas aeruginosa fumarate + NH3
-
r
L-phenylalanine
-
Pseudomonas aeruginosa cinnamic acid + NH3
-
r

Subunits

Subunits Comment Organism
? x * 51000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
L-aspartate ammonia lyase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
deamination reaction Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 15°C: about 80% of maximal activity, 30°C: about 40% of maximal activity, 35: maximal activity, 40°C: about 90% of maximal activity, 50°C: about 30% of maximal activity Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
the enzyme retains 56% activity after 7 days of incubation Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.908
-
L-aspartate pH 8.0, 35°C Pseudomonas aeruginosa
8.002
-
L-phenylalanine pH 8., 35°C Pseudomonas aeruginosa
13.64
-
fumarate pH 8.0, 35°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
deamination reaction Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 80% of maximal activity, pH 8.0: maximal activity, pH 9.0: about 25% of maximal activity, deamination reaction Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.038
-
fumarate pH 8.0, 35°C Pseudomonas aeruginosa
4.65
-
L-phenylalanine pH 8.0, 35°C Pseudomonas aeruginosa
14.18
-
L-aspartate pH 8.0, 35°C Pseudomonas aeruginosa