Literature summary for 4.3.1.1 extracted from

Bearden, S.W.; Sexton, C.; Pare, J.; Fowler, J.M.; Arvidson, C.G.; Yerman, L.; Viola, R.E.; Brubaker, R.R.
Attenuated enzootic (pestoides) isolates of Yersinia pestis express active aspartase (2009), Microbiology, 155, 198-209.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	included in assay medium	Yersinia pseudotuberculosis
Mg2+	included in assay medium	Yersinia pestis
Mg2+	included in assay medium	Yersinia enterocolitica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate	Yersinia pseudotuberculosis	-	fumarate + NH3	-	?
L-aspartate	Yersinia pestis	-	fumarate + NH3	-	?
L-aspartate	Yersinia enterocolitica	-	fumarate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Yersinia enterocolitica	-	-	-
Yersinia pestis	-	enzootic (pestoides) isolates A-D	-
Yersinia pestis	-	enzootic (pestoides) isolates E, F, G and I	-
Yersinia pestis	-	strains Angola and A16	-
Yersinia pseudotuberculosis	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Yersinia pseudotuberculosis	-
cell culture	-	Yersinia pestis	-
cell culture	-	Yersinia enterocolitica	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	full AspA activity occurs in pestoides isolates where valine (pestoides A, B, C and D) or serine (pestoides E, F, G and I) occupy position 363. Reduced activity occurs in strains Angola and A16, which contains phenylalanine at position 363. The kcat but not Km of purified AspA from strain Angola is significantly reduced.	Yersinia pestis
additional information	-	full AspA activity occurs in pestoides isolates where valine (pestoides A, B, C and D) or serine (pestoides E, F, G and I) occupy position 363. Reduced activity occurs in strains Angola and A16, which contains phenylalanine at this position. The kcat but not Km of purified AspA from strain Angola is significantly reduced.	Yersinia pestis
0.008	-	strain Angola	Yersinia pestis
0.02	-	strain A16	Yersinia pestis
0.028	-	pestoides D	Yersinia pestis
0.039	-	pestoides F	Yersinia pestis
0.042	-	pestoides B	Yersinia pestis
0.05	-	pestoides G	Yersinia pestis
0.05	-	pestoides I	Yersinia pestis
0.052	-	pestoides E	Yersinia pestis
0.057	-	pestoides C	Yersinia pestis
0.059	-	strain 7	Yersinia pseudotuberculosis
0.064	-	pestoides A	Yersinia pestis
0.072	-	strain TX83-0489	Yersinia pseudotuberculosis
0.137	-	strain PB1	Yersinia pseudotuberculosis
0.172	-	strain 1	Yersinia pseudotuberculosis
0.202	-	strain WA	Yersinia enterocolitica
0.202	-	strain Windblad	Yersinia enterocolitica
0.207	-	strain E705	Yersinia enterocolitica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate	-	Yersinia pseudotuberculosis	fumarate + NH3	-	?
L-aspartate	-	Yersinia pestis	fumarate + NH3	-	?
L-aspartate	-	Yersinia enterocolitica	fumarate + NH3	-	?

Synonyms

Synonyms	Comment	Organism
ASPA	-	Yersinia pseudotuberculosis
ASPA	-	Yersinia pestis
ASPA	-	Yersinia enterocolitica
aspartase	-	Yersinia pseudotuberculosis
aspartase	-	Yersinia pestis
aspartase	-	Yersinia enterocolitica
aspartate ammonia-lyase	-	Yersinia pseudotuberculosis
aspartate ammonia-lyase	-	Yersinia pestis
aspartate ammonia-lyase	-	Yersinia enterocolitica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Yersinia pseudotuberculosis
7	-	assay at	Yersinia pestis
7	-	assay at	Yersinia enterocolitica

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Release 2023.1 (January 2023)