Cloned (Comment) | Organism |
---|---|
functional and inactive aspA are cloned and expressed in AspA-deficient Escherichia coli | Yersinia pseudotuberculosis |
functional and inactive aspA of Yersinia pestis KIM is cloned and expressed in AspA-deficient Escherichia coli | Yersinia pestis |
Protein Variants | Comment | Organism |
---|---|---|
L363V | single mutant | Yersinia pestis |
V363L | mutation in primary structure causing dramatic differences in catalytic activity do not promote significant changes in secondary structure | Yersinia pseudotuberculosis |
Y146D | single mutant | Yersinia pestis |
Y146D/L363V | double mutant | Yersinia pestis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
L-aspartate | double mutant L363V/Y146D | Yersinia pestis | |
1.3 | - |
L-aspartate | mutant Y146D1 | Yersinia pestis | |
1.3 | - |
L-aspartate | recmbinant enzyme | Yersinia pestis | |
1.5 | - |
L-aspartate | mutant L363V | Yersinia pestis | |
2.1 | - |
L-aspartate | - |
Yersinia pseudotuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | included in assay medium | Yersinia pseudotuberculosis | |
Mg2+ | included in assay medium | Yersinia pestis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52470 | - |
mass of monomer, determined by an ESI-qTOF mass spectrometer | Yersinia pseudotuberculosis |
52540 | - |
mass of monomer, determined by an ESI-qTOF mass spectrometer | Yersinia pestis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Yersinia pseudotuberculosis | - |
fumarate + NH3 | fumarate is a component of the tricarboxylic acid (TCA) cycle | ? | |
L-aspartate | Yersinia pestis | - |
fumarate + NH3 | fumarate is a component of the tricarboxylic acid (TCA) cycle | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | - |
- |
- |
Yersinia pseudotuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
transformed cells of Escherichia coli JRG1476 are used to prepare the aspA products: proteins are fractionated in buffer with a NaCl gradient on successive anion-exchange Sepharose XL high-capacity and Source 30Q high-resolution chromatography columns | Yersinia pestis |
transformed cells of Escherichia coli JRG1476 are used to prepare the aspA products: proteins are fractionated in purification buffer with a NaCl gradient on successive anion-exchange Sepharose XL high-capacity and Source 30Q high-resolution chromatography columns | Yersinia pseudotuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain KIM, possesses a base transversion (GC-TA) at position 146 of AspA, causing replacement of aspartate by tyrosine | Yersinia pestis | - |
cell culture | strain PB1 of serogroup 1b | Yersinia pseudotuberculosis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity below 0.001, pCD- (lacking low-calcium response plasmid into pseudogenes) | Yersinia pestis |
additional information | - |
activity below 0.001, pCD- (lacking low-calcium response plasmid into pseudogenes), 4 mM Ca2+ | Yersinia pestis |
additional information | - |
activity below 0.001, pCD+ (containing low-calcium response plasmid into pseudogenes), 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 | Yersinia pestis |
additional information | - |
activity below 0.001, pCD+ (containing low-calcium response plasmid into pseudogenes), 4 mM Ca2+ | Yersinia pestis |
0.044 | - |
pYV- (lacking low-calcium response plasmid into pseudogenes), 4 mM Ca2+, 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 | Yersinia pseudotuberculosis |
0.05 | - |
pYV- (lacking low-calcium response plasmid into pseudogenes), 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 | Yersinia pseudotuberculosis |
0.06 | - |
pYV+ (containing low-calcium response plasmid into pseudogenes), 4 mM Ca2+, 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 | Yersinia pseudotuberculosis |
0.075 | - |
pYV+ (containing low-calcium response plasmid into pseudogenes), 250 mmol Tris/HCl buffer, 5.0 mmol MgCl2 | Yersinia pseudotuberculosis |
0.2 | - |
mutant L363V, 50 mM HEPES, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate | Yersinia pestis |
0.2 | - |
mutant Y146D, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate | Yersinia pestis |
0.2 | - |
recombinant enzyme, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate | Yersinia pestis |
72 | - |
double mutant L363V/Y146D, 50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate | Yersinia pestis |
80 | - |
50 mM HEPES, 10 mM Mg acetate, 20 mM L-aspartate | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Yersinia pseudotuberculosis | fumarate + NH3 | fumarate is a component of the tricarboxylic acid (TCA) cycle | ? | |
L-aspartate | - |
Yersinia pestis | fumarate + NH3 | fumarate is a component of the tricarboxylic acid (TCA) cycle | ? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | predicted and determined by dynamic light scattering (DLS), loss of activity in mutants reflects significant changes in subunit association. | Yersinia pestis |
Synonyms | Comment | Organism |
---|---|---|
ASPA | - |
Yersinia pseudotuberculosis |
ASPA | - |
Yersinia pestis |
aspartase | - |
Yersinia pseudotuberculosis |
aspartase | - |
Yersinia pestis |
aspartate ammonia-lyase | - |
Yersinia pseudotuberculosis |
aspartate ammonia-lyase | - |
Yersinia pestis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-aspartate | recombinant enzyme | Yersinia pestis | |
0.12 | - |
L-aspartate | mutant L363V | Yersinia pestis | |
0.13 | - |
L-aspartate | mutant Y146D | Yersinia pestis | |
86 | - |
L-aspartate | double mutant L363V/Y146D | Yersinia pestis | |
87 | - |
L-aspartate | - |
Yersinia pseudotuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
AspA assay in cell preparations | Yersinia pseudotuberculosis |
7 | - |
AspA assay in cell preparations | Yersinia pestis |
8 | - |
AspA assay in purified preparations | Yersinia pseudotuberculosis |
8 | - |
AspA assay in purified preparations | Yersinia pestis |